Source:http://linkedlifedata.com/resource/pubmed/id/20022123
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
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| pubmed:dateCreated |
2010-5-5
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| pubmed:abstractText |
F4(+) enterotoxigenic Escherichia coli (ETEC) infections are an important cause of postweaning diarrhoea in piglets and an oral immunization of piglets with purified F4 fimbriae protects them from a subsequent F4(+) ETEC infection. However, oral immunization of suckling piglets is hampered due to the immature status of their immune system. Targeting of antigens to Fcgamma receptors (FcgammaR) on human and murine dendritic cells (DC) has been shown to enhance DC maturation and both humoral and cellular immune responses. To investigate the effect of F4 fimbriae incorporated in immune complexes (F4-IC) on porcine DC, we used porcine monocytic-derived DC (MoDC) as a model system. The results in this study demonstrate that FcgammaRI, II and III mRNA is expressed by porcine MoDC. Furthermore, we show that FcgammaRII and III are expressed on the cell surface and that F4-IC are internalized by MoDC via FcgammaR. This FcgammaR ligation induced a significantly enhanced expression of Major Histocompatibility complex (MHCII) class II and the costimulatory molecules CD80/86 and CD40 by MoDC compared with immature MoDC. Furthermore, the phagocytic capacity of F4-IC stimulated MoDC was reduced as evidenced by a reduced uptake of DQ-ovalbumin and FITC-dextran. In an allogenic and autologous mixed lymphocyte reaction, these F4-IC-activated MoDC showed an improved T cell stimulatory capacity in comparison with immature MoDC. The F4-IC induced DC maturation correlated with significant higher expression levels of several pro-inflammatory cytokines such as interleukine (IL) 1beta, IL-6 and Tumor necrosis factor alpha, the chemokine IL-8 and IL-12p40 in comparison with immature MoDC. Altogether, these results clearly demonstrate that FcgammaR engagement enhances the maturation of porcine MoDC, which may suggest that antigen targeting to FcgammaR on DC could improve vaccine design against infections.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigen-Antibody Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Fimbriae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
1873-2534
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2009 Elsevier B.V. All rights reserved.
|
| pubmed:issnType |
Electronic
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| pubmed:day |
15
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| pubmed:volume |
135
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
188-98
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| pubmed:meshHeading |
pubmed-meshheading:20022123-Animals,
pubmed-meshheading:20022123-Antigen Presentation,
pubmed-meshheading:20022123-Antigen-Antibody Complex,
pubmed-meshheading:20022123-Antigens, Bacterial,
pubmed-meshheading:20022123-Base Sequence,
pubmed-meshheading:20022123-Cell Differentiation,
pubmed-meshheading:20022123-Cytokines,
pubmed-meshheading:20022123-DNA Primers,
pubmed-meshheading:20022123-Dendritic Cells,
pubmed-meshheading:20022123-Endocytosis,
pubmed-meshheading:20022123-Enterotoxigenic Escherichia coli,
pubmed-meshheading:20022123-Escherichia coli Infections,
pubmed-meshheading:20022123-Escherichia coli Vaccines,
pubmed-meshheading:20022123-Fimbriae, Bacterial,
pubmed-meshheading:20022123-Fimbriae Proteins,
pubmed-meshheading:20022123-Lymphocyte Activation,
pubmed-meshheading:20022123-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:20022123-Phagocytosis,
pubmed-meshheading:20022123-RNA, Messenger,
pubmed-meshheading:20022123-Receptors, IgG,
pubmed-meshheading:20022123-Sus scrofa,
pubmed-meshheading:20022123-Swine,
pubmed-meshheading:20022123-Swine Diseases
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| pubmed:year |
2010
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| pubmed:articleTitle |
Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
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| pubmed:affiliation |
Laboratory of Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. b.devriendt@Ugent.be
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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