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pubmed:abstractText |
Traditional chromatographic quantification methods for heparosan produced from the Escherichia coli K5 strain rely on extensive purification requiring laborious sample preparation. These methods are time-consuming, often resulting in sample loss during purification, and thus might not accurately reflect the amount of heparosan in the original mixture. A simple, sensitive (1)H nuclear magnetic resonance (NMR) quantification method that directly quantifies heparosan K5 polysaccharide present in E. coli fermentation supernatant is described.
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pubmed:affiliation |
Rensselaer Polytechnic Institute, Biotechnology Center, 110 8th Street, Troy, NY 12180-3590, USA. wangz2@rpi.edu
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