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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-4-18
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pubmed:databankReference |
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pubmed:abstractText |
The sequence and biochemical properties of the product of the cloned cDNA for the p65 subunit of nuclear factor kappa B (NF-kappa B) have been determined. The cDNA has an open reading frame of 549 amino acids capable of encoding a 60 kd protein. NF-kappa B p65 contains an amino-terminal region of 320 amino acids with extensive similarity to the oncogene c-rel and lesser similarity to NF-kappa B p50. In vitro translated p65 forms a DNA-binding complex with NF-kappa B p50, and the binding of this complex can be specifically inhibited by purified I kappa B. Progressive carboxy-terminal deletions of p65 show that, contrary to previous assumptions, p65 does include a DNA-binding domain that in vivo might become activated only through hetero-oligomerization with p50. DNA binding by truncated p65 is inhibited by I kappa B, thus mapping the I kappa B interaction domain to the rel-homologous region and suggesting that I kappa B exerts its inhibitory effect upon NF-kappa B primarily through interaction with p65.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
64
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pubmed:geneSymbol |
c-rel
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
961-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2001591-Amino Acid Sequence,
pubmed-meshheading:2001591-Animals,
pubmed-meshheading:2001591-Base Sequence,
pubmed-meshheading:2001591-Cloning, Molecular,
pubmed-meshheading:2001591-Macromolecular Substances,
pubmed-meshheading:2001591-Mice,
pubmed-meshheading:2001591-Models, Structural,
pubmed-meshheading:2001591-Molecular Sequence Data,
pubmed-meshheading:2001591-NF-kappa B,
pubmed-meshheading:2001591-Oligonucleotide Probes,
pubmed-meshheading:2001591-Open Reading Frames,
pubmed-meshheading:2001591-Polymerase Chain Reaction,
pubmed-meshheading:2001591-Protein Binding,
pubmed-meshheading:2001591-Protein Biosynthesis,
pubmed-meshheading:2001591-Protein Conformation,
pubmed-meshheading:2001591-Protein-Tyrosine Kinases,
pubmed-meshheading:2001591-Proto-Oncogene Proteins,
pubmed-meshheading:2001591-Proto-Oncogene Proteins c-rel,
pubmed-meshheading:2001591-Rabbits,
pubmed-meshheading:2001591-Recombinant Proteins,
pubmed-meshheading:2001591-Repressor Proteins,
pubmed-meshheading:2001591-Restriction Mapping,
pubmed-meshheading:2001591-Sequence Homology, Nucleic Acid
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pubmed:year |
1991
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pubmed:articleTitle |
DNA binding and I kappa B inhibition of the cloned p65 subunit of NF-kappa B, a rel-related polypeptide.
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pubmed:affiliation |
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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