|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2010-3-1
|
|
pubmed:abstractText |
Lin28 acts as a repressor of microRNA processing and as a post-transcriptional regulatory factor for a subset of mRNAs. Here we report that in human embryonic stem cells Lin28 facilitates the expression of the pivotal pluripotency factor Oct4 at the post-transcriptional level. We provide evidence that Lin28 binds Oct4 mRNA directly through high affinity sites within its coding region and that an interaction between Lin28 and RNA helicase A (RHA) may play a part in the observed regulation. We further demonstrate that decreasing RHA levels impairs Lin28-dependent stimulation of translation in a reporter system. Taken together with previous studies showing that RHA is required for efficient translation of a specific class of mRNAs, these findings suggest a novel mechanism by which Lin28 may affect target mRNA expression and represent the first evidence of post-transcriptional regulation of Oct4 expression by Lin28 in human embryonic stem cells.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-10742100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-11532944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-1408763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-14643679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-14688391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-16632596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-16680162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-17355893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-17382880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-17426138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-17473174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-17531811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-17679086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-18029452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-18292307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-18550544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-18566191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-18604195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-18725400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-18819918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-18951094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-19038789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-19147696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-19203577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-19443445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-19458619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-19483683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-19489092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-19703396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19966271-9054503
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DEAD-box RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DHX9 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/LIN-28 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-3,
http://linkedlifedata.com/resource/pubmed/chemical/POU5F1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
1362-4962
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
38
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1240-8
|
|
pubmed:dateRevised |
2010-9-27
|
|
pubmed:meshHeading |
pubmed-meshheading:19966271-Binding Sites,
pubmed-meshheading:19966271-Cell Line,
pubmed-meshheading:19966271-DEAD-box RNA Helicases,
pubmed-meshheading:19966271-Down-Regulation,
pubmed-meshheading:19966271-Embryonic Stem Cells,
pubmed-meshheading:19966271-Gene Expression Regulation,
pubmed-meshheading:19966271-Humans,
pubmed-meshheading:19966271-Neoplasm Proteins,
pubmed-meshheading:19966271-Octamer Transcription Factor-3,
pubmed-meshheading:19966271-Protein Biosynthesis,
pubmed-meshheading:19966271-RNA, Messenger,
pubmed-meshheading:19966271-RNA Interference,
pubmed-meshheading:19966271-RNA-Binding Proteins
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells.
|
|
pubmed:affiliation |
Yale Stem Cell Center, PO Box 208073, New Haven, CT 06520, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
