Source:http://linkedlifedata.com/resource/pubmed/id/19950171
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2010-2-3
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| pubmed:abstractText |
Controversy still surrounds the importance of cross-presentation versus endogenous or direct presentation of MHC-I restricted Ag in CD8(+) T-cell (T(CD8+)) immunity. It is even less clear what relative role these pathways play in shaping the T-cell repertoire specific for ubiquitous self-antigens, especially in cases where both Ag presentation pathways could potentially be involved. Here we provide evidence that a T(CD8+) repertoire specific for a determinant from the nuclear autoantigen La-SSB is largely shaped by direct presentation. In this system, mouse T(CD8+) reactive to a xenogeneic human La (hLa(51-58)) K(b) peptide did not recognize directly presented peptide on either spleen cells from hLa-Tg mice or hLa transfected syngeneic cells. Interestingly, the same T(CD8+) were activated by in vivo challenge with allogeneic APC expressing either the Tg hLa or loaded with intact recombinant hLa protein, indicating functional cross-presentation of the hLa(51-58). However, in irradiated bone marrow chimeric mice, DC expressing Tg hLa, but not WT DC that matured in hLa-Tg mice, constitutively presented the hLa(51-58) to T(CD8+). These data demonstrate that although both the direct- and cross-presentation pathways are potentially operative in revealing hLa(51-58) to T(CD8+), the T(CD8+) repertoire to this determinant is shaped quantitatively according to the efficiency of Ag presentation.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/H-2Kb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/SS-B antigen
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1521-4141
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
40
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
330-8
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| pubmed:meshHeading |
pubmed-meshheading:19950171-Amino Acid Sequence,
pubmed-meshheading:19950171-Animals,
pubmed-meshheading:19950171-Antigen Presentation,
pubmed-meshheading:19950171-Antigen-Presenting Cells,
pubmed-meshheading:19950171-Autoantigens,
pubmed-meshheading:19950171-CD8-Positive T-Lymphocytes,
pubmed-meshheading:19950171-Cell Line, Tumor,
pubmed-meshheading:19950171-Cross-Priming,
pubmed-meshheading:19950171-Dendritic Cells,
pubmed-meshheading:19950171-Female,
pubmed-meshheading:19950171-Flow Cytometry,
pubmed-meshheading:19950171-H-2 Antigens,
pubmed-meshheading:19950171-Humans,
pubmed-meshheading:19950171-Male,
pubmed-meshheading:19950171-Mice,
pubmed-meshheading:19950171-Mice, Inbred CBA,
pubmed-meshheading:19950171-Mice, Inbred Strains,
pubmed-meshheading:19950171-Mice, Transgenic,
pubmed-meshheading:19950171-Ribonucleoproteins,
pubmed-meshheading:19950171-Signal Transduction
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| pubmed:year |
2010
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| pubmed:articleTitle |
Direct antigen presentation by DC shapes the functional CD8(+) T-cell repertoire against the nuclear self-antigen La-SSB.
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| pubmed:affiliation |
Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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