Linked Life Data

19945433

Source:http://linkedlifedata.com/resource/pubmed/id/19945433

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-1-27
pubmed:abstractText
Transient receptor potential-melastatin 4 (TRPM4) is a Ca(2+)-activated, but Ca(2+)-impermeable, cation channel. Increasing [Ca(2+)](i) induce current activation and reduction through TRPM4 channels. Several TRPM4 isoforms are expressed in mice and humans, but rat TRPM4 (rTRPM4) has not been previously identified. Here, we identified, cloned, and characterized two rTRPM4 isoforms, rTRPM4a and rTRPM4b, using 5'-RACE-PCR. rTRPM4b channel activity increased with [Ca(2+)](i) in a dose-dependent manner. However, the rTRPM4b Ca(2+)-dependent activity at negative potentials differed from that of human TRPM4b (hTRPM4b), even though both represent full-length proteins. Additionally, rTRPM4b showed a slightly different single-channel current amplitude and open time distribution than hTRPM4b. However, rTRPM4a, which lacks the N-terminal region of rTRPM4b, and hTRPM4a had no similar functional channel activities. Furthermore, we characterized splicing regions, tissue distribution, and cellular localization of these isoforms. Unlike rTRPM4a, rTRPM4b was localized to the membrane at high levels, suggesting that rTRPM4b is the functionally active channel.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1090-2104
pubmed:author
pubmed:copyrightInfo
Crown Copyright 2009. Published by Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
391
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
806-11
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Cloning and characterization of rat transient receptor potential-melastatin 4 (TRPM4).
pubmed:affiliation
Department of Physiology, Institute of Health Science, and Medical Research Center for Neural Dysfunction, Biomedical Center (BK21), Gyeongsang National University College of Medicine, 90 Chilam-Dong, Jinju 660-751, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't