|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0025201,
umls-concept:C0031715,
umls-concept:C0056695,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0205171,
umls-concept:C0205245,
umls-concept:C0332157,
umls-concept:C0442805,
umls-concept:C1366876,
umls-concept:C1416655,
umls-concept:C1417172,
umls-concept:C1519755,
umls-concept:C1690540,
umls-concept:C1833235,
umls-concept:C2911684
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2010-4-29
|
|
pubmed:abstractText |
KIT is an essential receptor that modulates melanocyte function and whose function is disrupted in several pigmentary disorders. However, little is known about the effects of a single UVB exposure on the expression of KIT and two important regulatory transcription factors, MITF and AP-2 alpha, in human melanocytes. We found that a single UVB exposure of human melanocytes induces an early decrease and a subsequent increase in functional KIT expression in concert with up-regulated MITF expression. The increased MITF expression was accompanied by a markedly stimulated and prolonged phosphorylation of p38/CREB. The UVB-stimulated expression of KIT could be completely abolished by a p38 inhibitor, concomitant with a reduced phosphorylation of CREB and a down-regulation of MITF expression. Interestingly, in non-UVB exposed human melanocytes, a MEK inhibitor stimulated the phosphorylation of p38/CREB which was associated with an increased production of MITF and KIT in a pattern similar to that induced by UVB. These findings indicate that UVB stimulates functional KIT expression in human melanocytes via the up-regulation of MITF which is, in turn, due to the activation of p38 and CREB.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
1432-069X
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
302
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
283-94
|
|
pubmed:meshHeading |
pubmed-meshheading:19937254-Cells, Cultured,
pubmed-meshheading:19937254-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:19937254-Down-Regulation,
pubmed-meshheading:19937254-Humans,
pubmed-meshheading:19937254-MAP Kinase Kinase Kinases,
pubmed-meshheading:19937254-Melanocytes,
pubmed-meshheading:19937254-Microphthalmia-Associated Transcription Factor,
pubmed-meshheading:19937254-Phosphorylation,
pubmed-meshheading:19937254-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:19937254-Signal Transduction,
pubmed-meshheading:19937254-Ultraviolet Rays,
pubmed-meshheading:19937254-Up-Regulation,
pubmed-meshheading:19937254-p38 Mitogen-Activated Protein Kinases
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
A single UVB exposure increases the expression of functional KIT in human melanocytes by up-regulating MITF expression through the phosphorylation of p38/CREB.
|
|
pubmed:affiliation |
Department of Dermatology, Tokyo Women's Medical University, 8-1 Kawadacho, Shinjyuku, Tokyo, 162-8666, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
