Linked Life Data

19908751

Source:http://linkedlifedata.com/resource/pubmed/id/19908751

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2009-11-13
pubmed:abstractText
Bare and surface modified polystyrene nanoparticles with an anionic sulfate end group were synthesized by emulsion polymerization for adsorption of a protein (immunoglobulin G, IgG) by passive adsorption and electrostatic attraction for application as a biosensor. Dynamic light scattering (DLS) measurements confirmed the formation of an IgG-polystyrene latex complex, showing increased hydrodynamic diameter (D(h)) of approximately 178 nm (passive adsorption) and approximately 220 nm (electrostatic attraction) after IgG adsorption. An increased Zeta (zeta) potential value from -38.8 mV to -3.84 mV following IgG adsorption by electrostatic attraction also reflected the formation of the IgG-polystyrene latex complex. Solid phase immunodetection results demonstrated the formation of the IgG-polystyrene latex complex by showing fluorescent signals induced by FITC, which conjugated to IgG on the surface of the polystyrene. In addition, immunoassay results showed that adsorbed IgG on the polystyrene latex surface was not desorbed and maintained immunoreactivity after 1 month from the initial IgG-polystyrene latex complex formation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1533-4880
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7171-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Synthesis of anionic sulfate functionalized polystyrene latex and protein adsorption for application as a biosensor.
pubmed:affiliation
BioMEMS Team, Electronics and Telecommunication Research Institute, 131 Gajenong-Dong, Daejeon 305-700, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't