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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-1-4
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pubmed:abstractText |
Triggering receptor expressed on myeloid cells (TREM)-1 plays an important role in myeloid cell-activated inflammatory responses. Although TLR ligands such as LPS and lipoteichoic acid have been shown to upregulate TREM-1 expression in macrophage and neutrophils, the role of specific TLR in inducing the expression of TREM-1 remains unclear. In this study, we investigated whether the presence of TLR is necessary for the expression of TREM-1. We show that BM-derived macrophages from TLR4 and TLR2 KO mice failed to induce expression of TREM-1 message and protein in response to their specific ligands. Interestingly, the expression of TREM-1 in response to LPS is not altered in myeloid differentiation factor 88 (MyD88) KO macrophages, suggesting that downstream of TLR a MyD88-independent pathway induces the expression of TREM-1. Inhibiting toll/IL-1R domain-containing adaptor-inducing IFN-beta (TRIF) expression by siRNA decreased TREM-1 expression in response to LPS, suggesting that the expression of TREM-1 in response to LPS was mediated by the TRIF signaling pathway. On the other hand, the expression of TREM-1 in response to lipoteichoic acid is dependent on MyD88 expression. These data indicate that the expression of TREM-1 in response to TLR ligands occurs secondary to downstream signaling events and that the presence of TLR is necessary for the expression of TREM-1 in response to their specific ligands. However, the downstream signaling required for the expression of TREM-1 is dependent on the stimulus and the surface receptor through which the signaling is initiated.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Vesicular...,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Myd88 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Myeloid Differentiation Factor 88,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/TICAM-1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/TREM1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tlr2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tlr4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 4
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1521-4141
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
162-71
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pubmed:meshHeading |
pubmed-meshheading:19904768-Adaptor Proteins, Vesicular Transport,
pubmed-meshheading:19904768-Animals,
pubmed-meshheading:19904768-Cell Line,
pubmed-meshheading:19904768-Ligands,
pubmed-meshheading:19904768-Lipopolysaccharides,
pubmed-meshheading:19904768-Macrophages,
pubmed-meshheading:19904768-Membrane Glycoproteins,
pubmed-meshheading:19904768-Mice,
pubmed-meshheading:19904768-Mice, Inbred C57BL,
pubmed-meshheading:19904768-Mice, Knockout,
pubmed-meshheading:19904768-Mutation,
pubmed-meshheading:19904768-Myeloid Differentiation Factor 88,
pubmed-meshheading:19904768-RNA, Small Interfering,
pubmed-meshheading:19904768-Receptors, Immunologic,
pubmed-meshheading:19904768-Signal Transduction,
pubmed-meshheading:19904768-Substrate Specificity,
pubmed-meshheading:19904768-Toll-Like Receptor 2,
pubmed-meshheading:19904768-Toll-Like Receptor 4
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pubmed:year |
2010
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pubmed:articleTitle |
MYD88-dependent and -independent activation of TREM-1 via specific TLR ligands.
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pubmed:affiliation |
Department of Veterans Affairs, Jesse Brown VA Hospital, and Department of Pharmacology, University of Illinois, Chicago, IL 60612, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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