| pubmed-article:19895206 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19895206 | lifeskim:mentions | umls-concept:C0031809 | lld:lifeskim |
| pubmed-article:19895206 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:19895206 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:19895206 | lifeskim:mentions | umls-concept:C0026336 | lld:lifeskim |
| pubmed-article:19895206 | lifeskim:mentions | umls-concept:C0038250 | lld:lifeskim |
| pubmed-article:19895206 | lifeskim:mentions | umls-concept:C2930676 | lld:lifeskim |
| pubmed-article:19895206 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:19895206 | lifeskim:mentions | umls-concept:C0444925 | lld:lifeskim |
| pubmed-article:19895206 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:19895206 | pubmed:dateCreated | 2010-4-6 | lld:pubmed |
| pubmed-article:19895206 | pubmed:abstractText | Limited autologous vascular graft availability and poor patency rates of synthetic grafts for bypass or replacement of small-diameter arteries remain a concern in the surgical community. These limitations could potentially be improved by a tissue engineering approach. We report here our progress in the development and in vivo testing of a stem-cell-based tissue-engineered vascular graft for arterial applications. Poly(ester urethane)urea scaffolds (length = 10 mm; inner diameter = 1.2 mm) were created by thermally induced phase separation (TIPS). Compound scaffolds were generated by reinforcing TIPS scaffolds with an outer electrospun layer of the same biomaterial (ES-TIPS). Both TIPS and ES-TIPS scaffolds were bulk-seeded with 10 x 10(6) allogeneic, LacZ-transfected, muscle-derived stem cells (MDSCs), and then placed in spinner flask culture for 48 h. Constructs were implanted as interposition grafts in the abdominal aorta of rats for 8 weeks. Angiograms and histological assessment were performed at the time of explant. Cell-seeded constructs showed a higher patency rate than the unseeded controls: 65% (ES-TIPS) and 53% (TIPS) versus 10% (acellular TIPS). TIPS scaffolds had a 50% mechanical failure rate with aneurysmal formation, whereas no dilation was observed in the hybrid scaffolds. A smooth-muscle-like layer of cells was observed near the luminal surface of the constructs that stained positive for smooth muscle alpha-actin and calponin. LacZ+ cells were shown to be engrafted in the remodeled construct. A confluent layer of von Willebrand Factor-positive cells was observed in the lumen of MDSC-seeded constructs, whereas acellular controls showed platelet and fibrin deposition. This is the first evidence that MDSCs improve patency and contribute to the remodeling of a tissue-engineered vascular graft for arterial applications. | lld:pubmed |
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| pubmed-article:19895206 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19895206 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19895206 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19895206 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:19895206 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19895206 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:19895206 | pubmed:issn | 1937-335X | lld:pubmed |
| pubmed-article:19895206 | pubmed:author | pubmed-author:NieponiceAlej... | lld:pubmed |
| pubmed-article:19895206 | pubmed:author | pubmed-author:IOVAAA | lld:pubmed |
| pubmed-article:19895206 | pubmed:author | pubmed-author:HuardJohnnyJ | lld:pubmed |
| pubmed-article:19895206 | pubmed:author | pubmed-author:WagnerWilliam... | lld:pubmed |
| pubmed-article:19895206 | pubmed:author | pubmed-author:GuanJianjunJ | lld:pubmed |
| pubmed-article:19895206 | pubmed:author | pubmed-author:VorpDavid ADA | lld:pubmed |
| pubmed-article:19895206 | pubmed:author | pubmed-author:MaulTimothy... | lld:pubmed |
| pubmed-article:19895206 | pubmed:author | pubmed-author:GharaibehBurh... | lld:pubmed |
| pubmed-article:19895206 | pubmed:author | pubmed-author:SolettiLorenz... | lld:pubmed |
| pubmed-article:19895206 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19895206 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:19895206 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19895206 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19895206 | pubmed:pagination | 1215-23 | lld:pubmed |
| pubmed-article:19895206 | pubmed:dateRevised | 2011-7-27 | lld:pubmed |
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| pubmed-article:19895206 | pubmed:meshHeading | pubmed-meshheading:19895206... | lld:pubmed |
| pubmed-article:19895206 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:19895206 | pubmed:articleTitle | In vivo assessment of a tissue-engineered vascular graft combining a biodegradable elastomeric scaffold and muscle-derived stem cells in a rat model. | lld:pubmed |
| pubmed-article:19895206 | pubmed:affiliation | Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh , Pittsburgh, PA, USA. | lld:pubmed |
| pubmed-article:19895206 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19895206 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19895206 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| pubmed-article:19895206 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
