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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2010-4-6
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pubmed:abstractText |
Limited autologous vascular graft availability and poor patency rates of synthetic grafts for bypass or replacement of small-diameter arteries remain a concern in the surgical community. These limitations could potentially be improved by a tissue engineering approach. We report here our progress in the development and in vivo testing of a stem-cell-based tissue-engineered vascular graft for arterial applications. Poly(ester urethane)urea scaffolds (length = 10 mm; inner diameter = 1.2 mm) were created by thermally induced phase separation (TIPS). Compound scaffolds were generated by reinforcing TIPS scaffolds with an outer electrospun layer of the same biomaterial (ES-TIPS). Both TIPS and ES-TIPS scaffolds were bulk-seeded with 10 x 10(6) allogeneic, LacZ-transfected, muscle-derived stem cells (MDSCs), and then placed in spinner flask culture for 48 h. Constructs were implanted as interposition grafts in the abdominal aorta of rats for 8 weeks. Angiograms and histological assessment were performed at the time of explant. Cell-seeded constructs showed a higher patency rate than the unseeded controls: 65% (ES-TIPS) and 53% (TIPS) versus 10% (acellular TIPS). TIPS scaffolds had a 50% mechanical failure rate with aneurysmal formation, whereas no dilation was observed in the hybrid scaffolds. A smooth-muscle-like layer of cells was observed near the luminal surface of the constructs that stained positive for smooth muscle alpha-actin and calponin. LacZ+ cells were shown to be engrafted in the remodeled construct. A confluent layer of von Willebrand Factor-positive cells was observed in the lumen of MDSC-seeded constructs, whereas acellular controls showed platelet and fibrin deposition. This is the first evidence that MDSCs improve patency and contribute to the remodeling of a tissue-engineered vascular graft for arterial applications.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19895206-10205057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19895206-10880112,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1937-335X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1215-23
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pubmed:dateRevised |
2011-7-27
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pubmed:meshHeading |
pubmed-meshheading:19895206-Adult Stem Cells,
pubmed-meshheading:19895206-Animals,
pubmed-meshheading:19895206-Aorta, Abdominal,
pubmed-meshheading:19895206-Biocompatible Materials,
pubmed-meshheading:19895206-Blood Vessel Prosthesis,
pubmed-meshheading:19895206-Elastomers,
pubmed-meshheading:19895206-Lac Operon,
pubmed-meshheading:19895206-Microscopy, Electron, Scanning,
pubmed-meshheading:19895206-Myocytes, Smooth Muscle,
pubmed-meshheading:19895206-Polyesters,
pubmed-meshheading:19895206-Rats,
pubmed-meshheading:19895206-Rats, Inbred Lew,
pubmed-meshheading:19895206-Tissue Engineering,
pubmed-meshheading:19895206-Tissue Scaffolds,
pubmed-meshheading:19895206-Transfection,
pubmed-meshheading:19895206-Transplantation, Homologous
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pubmed:year |
2010
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pubmed:articleTitle |
In vivo assessment of a tissue-engineered vascular graft combining a biodegradable elastomeric scaffold and muscle-derived stem cells in a rat model.
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pubmed:affiliation |
Department of Surgery, McGowan Institute for Regenerative Medicine, University of Pittsburgh , Pittsburgh, PA, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies,
Research Support, N.I.H., Extramural
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