Linked Life Data

19876843

Source:http://linkedlifedata.com/resource/pubmed/id/19876843

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-12-16
pubmed:abstractText
We have previously shown that mouse embryonic stem (ES) cells differentiate into insulin-positive cells via multi-lineage progenitors. Here, we used Affymetrix chips and quantitative RT-PCR analysis to determine transcriptional profiles of undifferentiated wildtype (wt) and Pax4 expressing (Pax4+) ES cells and differentiated cells of committed progenitor and advanced stages. From undifferentiated to the committed stage, 237 (wt) and 263 (Pax4+) transcripts were 5- or more-fold up-regulated, whereas from the committed to the advanced stage, 28 (wt) and 5 (Pax4+) transcripts, respectively, were two- or more-fold up-regulated. Transcripts were classified into main subclasses including transcriptional regulation, signalling/growth factors, adhesion/extracellular matrix, membrane/transport, metabolism and organogenesis. Remarkably, endoderm-specific Sox17 and early pancreas-specific Isl1 transcripts were up-regulated at an earlier stage of multi-lineage progenitors, whereas highly up-regulated probe sets and transcripts of genes involved in endoderm, pancreatic, hepatic, angiogenic and neural differentiation were detected at the committed progenitor stage. Pax4+ cells showed specific differences in transcript up-regulation and a lower amount of up-regulated neural-specific transcripts in comparison to wt cells, but no enhanced gene expression complexity. Immunocytochemical analysis of selected proteins involved in endoderm and pancreatic differentiation, such as chromogranin B, transthyretin, Foxa1 and neuronatin revealed co-expression with insulin- or C-peptide-positive cells. The comparison of transcript profiles of ES cells differentiating in vitro with those of the embryonic and adult pancreas in vivo suggested that in vitro differentiated cells resemble an embryonal stage of development, supporting the view that ES-derived pancreatic cells are unable to complete pancreatic differentiation in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1696-3547
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-54
pubmed:meshHeading
pubmed-meshheading:19876843-Animals, pubmed-meshheading:19876843-Biological Markers, pubmed-meshheading:19876843-Blotting, Western, pubmed-meshheading:19876843-Cell Differentiation, pubmed-meshheading:19876843-Cell Lineage, pubmed-meshheading:19876843-Cells, Cultured, pubmed-meshheading:19876843-Embryo, Mammalian, pubmed-meshheading:19876843-Embryonic Stem Cells, pubmed-meshheading:19876843-Fluorescent Antibody Technique, pubmed-meshheading:19876843-Gene Expression Profiling, pubmed-meshheading:19876843-Gene Expression Regulation, Developmental, pubmed-meshheading:19876843-Homeodomain Proteins, pubmed-meshheading:19876843-Immunoenzyme Techniques, pubmed-meshheading:19876843-Insulin-Secreting Cells, pubmed-meshheading:19876843-Mice, pubmed-meshheading:19876843-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:19876843-Paired Box Transcription Factors, pubmed-meshheading:19876843-Pancreas, pubmed-meshheading:19876843-RNA, Messenger, pubmed-meshheading:19876843-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2010
pubmed:articleTitle
Characterization of mouse embryonic stem cell differentiation into the pancreatic lineage in vitro by transcriptional profiling, quantitative RT-PCR and immunocytochemistry.
pubmed:affiliation
In vitro Differentiation Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't