Linked Life Data

19874716

Source:http://linkedlifedata.com/resource/pubmed/id/19874716

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-10-30
pubmed:abstractText
The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1976-6696
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
691-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells.
pubmed:affiliation
Department of Biology, Kyung Hee University, Seoul, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't