Linked Life Data

19857067

Source:http://linkedlifedata.com/resource/pubmed/id/19857067

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-10-27
pubmed:abstractText
Our previous study demonstrated that methanolic extract of Inula helenium (Elecampane) has the potential to induce detoxifying enzymes such as NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) (NQO1, QR) activity and glutathione S-transferase (GST) and found isoalantolactone and alantolactone as the active components. In this study we investigated the detoxifying enzyme-inducing potential of isoalantolactone, which is present in I. helenium and has a structure similar to that of alantolactone. The compound induced QR in a dose-dependent manner in both Hepa1c1c7 cells and its mutant BPRc1 cells lacking the arylhydrocarbon receptor translocator. Like with most phase 2 enzyme inducers, other phase 2 detoxifying enzymes, including GST, glutathione reductase, gamma-glutamylcysteine synthetase, and heme oxygenase-1, were also induced by isoalantolactone in a dose-dependent manner in the cultured cells. Furthermore, isoalantolactone caused a proportionate increase in luciferase activity depending upon concentration and exposure time in the reporter assay in which HepG2-C8 cells, transfectants carrying antioxidant response element-luciferase gene, were used. The nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) was stimulated by the compound and attenuated by phosphatidylinositol 3-kinase inhibitors such as LY294002 and wortmannin. In conclusion, isoalantolactone is a candidate for chemoprevention and acts as potent phase 2 enzyme inducer by stimulating the accumulation of Nrf2 in the nucleus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1557-7600
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1038-45
pubmed:meshHeading
pubmed-meshheading:19857067-Animals, pubmed-meshheading:19857067-Antineoplastic Agents, Phytogenic, pubmed-meshheading:19857067-Cell Line, Tumor, pubmed-meshheading:19857067-Dose-Response Relationship, Drug, pubmed-meshheading:19857067-Enzymes, pubmed-meshheading:19857067-Fruit, pubmed-meshheading:19857067-Hep G2 Cells, pubmed-meshheading:19857067-Humans, pubmed-meshheading:19857067-Inula, pubmed-meshheading:19857067-Liver Neoplasms, pubmed-meshheading:19857067-Metabolic Detoxication, Drug, pubmed-meshheading:19857067-Mice, pubmed-meshheading:19857067-NAD(P)H Dehydrogenase (Quinone), pubmed-meshheading:19857067-NF-E2-Related Factor 2, pubmed-meshheading:19857067-Phytotherapy, pubmed-meshheading:19857067-Plant Extracts, pubmed-meshheading:19857067-Plant Roots, pubmed-meshheading:19857067-Sesquiterpenes
pubmed:year
2009
pubmed:articleTitle
Isoalantolactone from Inula helenium caused Nrf2-mediated induction of detoxifying enzymes.
pubmed:affiliation
Department of Animal Science and Biotechnology and School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't