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rdf:type |
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lifeskim:mentions |
umls-concept:C0006826,
umls-concept:C0042333,
umls-concept:C0205314,
umls-concept:C0332466,
umls-concept:C0599894,
umls-concept:C0679622,
umls-concept:C1511790,
umls-concept:C1519595,
umls-concept:C1521840,
umls-concept:C1561491,
umls-concept:C1956267,
umls-concept:C2349975
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pubmed:issue |
10
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pubmed:dateCreated |
2009-11-27
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pubmed:abstractText |
Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-11125122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-11381028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-12529310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-14973331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-14993899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-15361874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-17348159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-17934467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-17934468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-17934469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-17982454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-18438408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-18516045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-18519653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-18599465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-18772439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-18772890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-18987734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19015660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19056696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19136943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19166587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19181860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19182786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19208812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19349981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19410533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19478186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19543287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19592507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-19620972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-7769849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19835606-7937745
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1465-6914
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
R115
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pubmed:dateRevised |
2011-6-30
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pubmed:meshHeading |
pubmed-meshheading:19835606-Amino Acid Sequence,
pubmed-meshheading:19835606-Base Sequence,
pubmed-meshheading:19835606-DNA, Complementary,
pubmed-meshheading:19835606-Gene Expression Profiling,
pubmed-meshheading:19835606-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:19835606-Humans,
pubmed-meshheading:19835606-K562 Cells,
pubmed-meshheading:19835606-Molecular Sequence Data,
pubmed-meshheading:19835606-Mutation,
pubmed-meshheading:19835606-Neoplasms,
pubmed-meshheading:19835606-Oncogene Proteins, Fusion,
pubmed-meshheading:19835606-RNA, Messenger,
pubmed-meshheading:19835606-Sequence Analysis, DNA
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pubmed:year |
2009
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pubmed:articleTitle |
Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts.
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pubmed:affiliation |
Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 02141, USA. jlevin@broadinstitute.org
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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