| pubmed-article:1981801 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1981801 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:1981801 | lifeskim:mentions | umls-concept:C0003320 | lld:lifeskim |
| pubmed-article:1981801 | lifeskim:mentions | umls-concept:C1427539 | lld:lifeskim |
| pubmed-article:1981801 | lifeskim:mentions | umls-concept:C0449557 | lld:lifeskim |
| pubmed-article:1981801 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:1981801 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1981801 | pubmed:dateCreated | 1991-4-25 | lld:pubmed |
| pubmed-article:1981801 | pubmed:abstractText | Production of coli surface-associated antigen 1 (CS1) by Escherichia coli strain E24377 of serotype O139.H28 was controlled by a plasmid that also encoded heat stable and heat labile enterotoxins and CS3. The presence of a regulatory sequence was detected on this plasmid by hybridization with the cfaD gene that regulates expression of colonization factor antigen I fimbriae and is at least 96% homologous with the rns sequence controlling production of CS1 or CS2 fimbriae by strains of serotype O6.H16 of appropriate biotype. A separate plasmid, pDEP20, carrying the structural genes for CS1 synthesis was identified and transformed into E. coli strain HB101 or a derivative of strain E24377 without large plasmids. Transformants carrying pDEP20 did not produce CS1 fimbrial antigen, but antigen expression was obtained when a cloned cfaD gene or a wild-type plasmid carrying the rns sequence was introduced. Transposon mutagenesis with Tn1000 identified a 3.7 kbp region of pDEP20 essential for production of CS1 fimbriae. Genes encoding production of CS1 fimbriae were cloned on a 9.9 kbp BamHI fragment and were expressed in the presence of the cfaD sequence. A strain producing both CS1 and CS2 antigens was constructed by introduction of the cloned cfaD gene into a strain of serotype O6.H16 biotype C carrying plasmid pDEP20. | lld:pubmed |
| pubmed-article:1981801 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1981801 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1981801 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1981801 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1981801 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1981801 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1981801 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1981801 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1981801 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1981801 | pubmed:issn | 0882-4010 | lld:pubmed |
| pubmed-article:1981801 | pubmed:author | pubmed-author:GaastraWW | lld:pubmed |
| pubmed-article:1981801 | pubmed:author | pubmed-author:ThomasAA | lld:pubmed |
| pubmed-article:1981801 | pubmed:author | pubmed-author:RoweBB | lld:pubmed |
| pubmed-article:1981801 | pubmed:author | pubmed-author:SmithH RHR | lld:pubmed |
| pubmed-article:1981801 | pubmed:author | pubmed-author:McConnellM... | lld:pubmed |
| pubmed-article:1981801 | pubmed:author | pubmed-author:WillshawG AGA | lld:pubmed |
| pubmed-article:1981801 | pubmed:author | pubmed-author:HibberdMM | lld:pubmed |
| pubmed-article:1981801 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1981801 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:1981801 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1981801 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1981801 | pubmed:pagination | 1-11 | lld:pubmed |
| pubmed-article:1981801 | pubmed:dateRevised | 2006-5-1 | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:meshHeading | pubmed-meshheading:1981801-... | lld:pubmed |
| pubmed-article:1981801 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:1981801 | pubmed:articleTitle | Plasmid-encoded production of coli surface-associated antigen 1 (CS1) in a strain of Escherichia coli serotype O139.H28. | lld:pubmed |
| pubmed-article:1981801 | pubmed:affiliation | Division of Enteric Pathogens, Central Public Health Laboratory, London, U.K. | lld:pubmed |
| pubmed-article:1981801 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1981801 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1981801 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1981801 | lld:pubmed |
