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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1991-4-25
|
| pubmed:abstractText |
Production of coli surface-associated antigen 1 (CS1) by Escherichia coli strain E24377 of serotype O139.H28 was controlled by a plasmid that also encoded heat stable and heat labile enterotoxins and CS3. The presence of a regulatory sequence was detected on this plasmid by hybridization with the cfaD gene that regulates expression of colonization factor antigen I fimbriae and is at least 96% homologous with the rns sequence controlling production of CS1 or CS2 fimbriae by strains of serotype O6.H16 of appropriate biotype. A separate plasmid, pDEP20, carrying the structural genes for CS1 synthesis was identified and transformed into E. coli strain HB101 or a derivative of strain E24377 without large plasmids. Transformants carrying pDEP20 did not produce CS1 fimbrial antigen, but antigen expression was obtained when a cloned cfaD gene or a wild-type plasmid carrying the rns sequence was introduced. Transposon mutagenesis with Tn1000 identified a 3.7 kbp region of pDEP20 essential for production of CS1 fimbriae. Genes encoding production of CS1 fimbriae were cloned on a 9.9 kbp BamHI fragment and were expressed in the presence of the cfaD sequence. A strain producing both CS1 and CS2 antigens was constructed by introduction of the cloned cfaD gene into a strain of serotype O6.H16 biotype C carrying plasmid pDEP20.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0882-4010
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1-11
|
| pubmed:dateRevised |
2006-5-1
|
| pubmed:meshHeading |
pubmed-meshheading:1981801-Antigens, Bacterial,
pubmed-meshheading:1981801-Cloning, Molecular,
pubmed-meshheading:1981801-DNA Transposable Elements,
pubmed-meshheading:1981801-Escherichia coli,
pubmed-meshheading:1981801-Fimbriae, Bacterial,
pubmed-meshheading:1981801-Fimbriae Proteins,
pubmed-meshheading:1981801-Gene Expression Regulation, Bacterial,
pubmed-meshheading:1981801-Plasmids,
pubmed-meshheading:1981801-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1981801-Restriction Mapping,
pubmed-meshheading:1981801-Serotyping,
pubmed-meshheading:1981801-Transformation, Bacterial
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Plasmid-encoded production of coli surface-associated antigen 1 (CS1) in a strain of Escherichia coli serotype O139.H28.
|
| pubmed:affiliation |
Division of Enteric Pathogens, Central Public Health Laboratory, London, U.K.
|
| pubmed:publicationType |
Journal Article
|