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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
3
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pubmed:dateCreated |
1991-3-25
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pubmed:abstractText |
Two nonallelic mouse mutations with severe dwarf phenotypes are characterized by a lack of growth hormone, prolactin, and thyroid stimulating hormone. The cells that normally synthesize these pituitary hormones express a common transcription factor called GHF-1 or Pit-1. Using an intersubspecific backcross, we have demonstrated tight linkage of the Pit-1 and Snell dwarf (dw) genes on mouse chromosome 16. No recombination was observed between Pit-1 and dw in 110 individuals examined. Southern blot analysis of genomic DNA reveals that the Pit-1 gene is rearranged in C3H/HeJ-dwJ/dw mice but not in coisogenic +/+ animals, providing molecular evidence that a lesion in the Pit-1 gene results in the Snell dwarf phenotype. Demonstration of low levels of Pit-1 expression in Ames dwarf (df) mice implies that both Pit-1 and df expression may be required for pituitary differentiation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0888-7543
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:geneSymbol |
df,
dw,
dw<up>J</up>
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
586-90
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:1981057-Animals,
pubmed-meshheading:1981057-Cell Differentiation,
pubmed-meshheading:1981057-Crosses, Genetic,
pubmed-meshheading:1981057-DNA Mutational Analysis,
pubmed-meshheading:1981057-DNA-Binding Proteins,
pubmed-meshheading:1981057-Dwarfism, Pituitary,
pubmed-meshheading:1981057-Gene Expression Regulation,
pubmed-meshheading:1981057-Genes, Homeobox,
pubmed-meshheading:1981057-Growth Hormone,
pubmed-meshheading:1981057-Mice,
pubmed-meshheading:1981057-Mice, Inbred C3H,
pubmed-meshheading:1981057-Mice, Inbred Strains,
pubmed-meshheading:1981057-Mice, Mutant Strains,
pubmed-meshheading:1981057-Pituitary Gland, Anterior,
pubmed-meshheading:1981057-Prolactin,
pubmed-meshheading:1981057-Recombination, Genetic,
pubmed-meshheading:1981057-Thyrotropin,
pubmed-meshheading:1981057-Transcription Factor Pit-1,
pubmed-meshheading:1981057-Transcription Factors
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pubmed:year |
1990
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pubmed:articleTitle |
The Pit-1 transcription factor gene is a candidate for the murine Snell dwarf mutation.
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pubmed:affiliation |
Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109-0618.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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