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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2009-10-5
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pubmed:abstractText |
The fission yeast Schizosaccharomyces pombe has many biological characteristics that make it an ideal model organism for the study of meiosis. A nearly synchronous meiosis is one of the most important. Under certain environmental and genetic conditions, large cultures of S. pombe can be induced to undergo meiosis in a timely and predictable manner that allows for changes in the DNA to be observed and analyzed by gel electrophoresis. Initiation of meiotic recombination via programmed DNA double-strand breaks, the formation of joint molecule recombination intermediates, and the resolution of these intermediates into crossover DNA products can all be seen with consistent timing during the progression of a synchronous meiotic induction. The timing of recombination events, the genetic requirements for the formation and disappearance of recombination intermediates, and the analysis of the DNA structures of those intermediates allow a comparison of meiotic recombination in fission yeast with that in the only other species similarly studied, the budding yeast Saccharomyces cerevisiae.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-10882124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-11529427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-11864600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-12019258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-17174892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-17722984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-1996103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-2822257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-3870979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-8287479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19799186-8468345
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1064-3745
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
557
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
235-52
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pubmed:dateRevised |
2010-12-3
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pubmed:meshHeading |
pubmed-meshheading:19799186-Cell Culture Techniques,
pubmed-meshheading:19799186-DNA, Fungal,
pubmed-meshheading:19799186-Electrophoresis, Agar Gel,
pubmed-meshheading:19799186-Meiosis,
pubmed-meshheading:19799186-Models, Biological,
pubmed-meshheading:19799186-Recombination, Genetic,
pubmed-meshheading:19799186-Schizosaccharomyces
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pubmed:year |
2009
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pubmed:articleTitle |
Using Schizosaccharomyces pombe meiosis to analyze DNA recombination intermediates.
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pubmed:affiliation |
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
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pubmed:publicationType |
Journal Article,
Review,
Research Support, N.I.H., Extramural
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