Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-10-2
pubmed:abstractText
To examine transcription factor (TF) network(s), we created mouse ESC lines, in each of which 1 of 50 TFs tagged with a FLAG moiety is inserted into a ubiquitously controllable tetracycline-repressible locus. Of the 50 TFs, Cdx2 provoked the most extensive transcriptome perturbation in ESCs, followed by Esx1, Sox9, Tcf3, Klf4, and Gata3. ChIP-Seq revealed that CDX2 binds to promoters of upregulated target genes. By contrast, genes downregulated by CDX2 did not show CDX2 binding but were enriched with binding sites for POU5F1, SOX2, and NANOG. Genes with binding sites for these core TFs were also downregulated by the induction of at least 15 other TFs, suggesting a common initial step for ESC differentiation mediated by interference with the binding of core TFs to their target genes. These ESC lines provide a fundamental resource to study biological networks in ESCs and mice.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix..., http://linkedlifedata.com/resource/pubmed/chemical/Cdx2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Esx1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/GATA3 Transcription Factor, http://linkedlifedata.com/resource/pubmed/chemical/GKLF protein, http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Kruppel-Like Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/SOX9 Transcription Factor, http://linkedlifedata.com/resource/pubmed/chemical/Sox9 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Tcf3 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1875-9777
pubmed:author
pubmed-author:BasseyUwemU, pubmed-author:Correa-CerroLina SLS, pubmed-author:DudekulaDawoodD, pubmed-author:FalcoGeppinoG, pubmed-author:GoldbergIlyaI, pubmed-author:HoangHienH, pubmed-author:IslamMd NurulMN, pubmed-author:KimEugeneE, pubmed-author:KoMinoru S HMS, pubmed-author:LeeSung-LimSL, pubmed-author:LiManxiangM, pubmed-author:LongoDan LDL, pubmed-author:MarshallThomasT, pubmed-author:MehtaSamirS, pubmed-author:MeyersEmilyE, pubmed-author:MontiManuelaM, pubmed-author:MowrerGregoryG, pubmed-author:NagarajaRamaiahR, pubmed-author:NakatakeYuhkiY, pubmed-author:NishiyamaAkiraA, pubmed-author:PiaoYulanY, pubmed-author:QianYongY, pubmed-author:SchlessingerDavidD, pubmed-author:SharovAlexei AAA, pubmed-author:SharovaLioudmilaL, pubmed-author:StaggCaroleC, pubmed-author:StanghelliniIlariaI, pubmed-author:TapnioRichardR, pubmed-author:WangWeidongW, pubmed-author:XuZ CZC, pubmed-author:YangHsih-TeHT, pubmed-author:YeeSarahS, pubmed-author:ZalzmanMichalM
pubmed:issnType
Electronic
pubmed:day
2
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
420-33
pubmed:dateRevised
2010-12-3
pubmed:meshHeading
pubmed-meshheading:19796622-Animals, pubmed-meshheading:19796622-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:19796622-Cell Differentiation, pubmed-meshheading:19796622-Cell Line, pubmed-meshheading:19796622-Chromatin Immunoprecipitation, pubmed-meshheading:19796622-Embryonic Stem Cells, pubmed-meshheading:19796622-GATA3 Transcription Factor, pubmed-meshheading:19796622-Gene Regulatory Networks, pubmed-meshheading:19796622-Homeodomain Proteins, pubmed-meshheading:19796622-Immunoprecipitation, pubmed-meshheading:19796622-Kruppel-Like Transcription Factors, pubmed-meshheading:19796622-Mice, pubmed-meshheading:19796622-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:19796622-Proto-Oncogene Proteins, pubmed-meshheading:19796622-SOX9 Transcription Factor, pubmed-meshheading:19796622-Transcription Factors
pubmed:year
2009
pubmed:articleTitle
Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors.
pubmed:affiliation
National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
pubmed:publicationType
Journal Article