Linked Life Data

19781808

Source:http://linkedlifedata.com/resource/pubmed/id/19781808

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-2-26
pubmed:abstractText
A soft micropillar extracellular environment of distinct biomechanics is established by fabricating polydimethylsiloxane (PDMS) interfaces with pillar distances of 5, 7, 9 and 11 microm and elasticity moduli of 0.6, 1.0 and 3.5 Mega Pascal. To allow for cell adhesion, the biomimetic concept of pillar head fibronectin (FN) biofunctionalisation is applied. This environmental set-up aims at the analysis of favourable conditions for cell behaviour of three periodontal cell-types, here reflected by the establishment of regular cell morphology and optimal collagen gene expression. Biomechanics of these predefined functionalized model surfaces reveal progressive deterioration of regular cell morphology with increasing pillar distance, independent from pillar elasticity and cell type. Analysis of collagen gene expression demonstrates interdependency to the elasticity and the micropattern of the extracellular environment in all cell types under study. The results suggest that biomechanics of the extracellular environment govern tissue-specific cell behaviour in different periodontal cell types. Moreover, they form the basis for the creation of new biomaterials which address distinct cell functions by specific biomechanical properties.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1618-1298
pubmed:author
pubmed:copyrightInfo
(c) 2009 Elsevier GmbH. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-25
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Soft micropillar interfaces of distinct biomechanics govern behaviour of periodontal cells.
pubmed:affiliation
Department of Oral Biotechnology, Dental School, University of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't