Linked Life Data

19772286

Source:http://linkedlifedata.com/resource/pubmed/id/19772286

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2009-10-14
pubmed:abstractText
Simultaneous determination of wild-type and total p53 proteins (wild-type and mutant combined) present in cancer cell lysates has been performed with a dual-channel surface plasmon resonance (SPR) instrument. To achieve specificity, each channel of the SPR chip was modified with a consensus double-stranded (ds-) DNA and a monoclonal antibody. The high affinity of the consensus ds-DNA to the wild-type p53 and the antibody to total p53 results in remarkably low detection levels (10.6 and 1.06 pM for the wild-type and total p53, respectively). The difference between the SPR signals reveals the extent of p53 mutation, which is indicative of cancer development. The SPR signals increase with the p53 concentration across a wide range (from low picomolar to nanomolar levels) that amply encompasses the typical cellular p53 concentrations. The applicability of the method to real sample analysis has been demonstrated with the comparative analyses of normal and cancer cell lysates. The normal cell samples all displayed significantly higher levels of wild-type p53. In contrast, elevated levels of mutant p53 were observed from the cancer cell lysates. In comparison with enzyme-linked immunosorbant assay (ELISA), SPR obviates the need of a second antibody labeled with an enzyme in the "sandwich enzyme immunoassay" format and is capable of real-time monitoring of the binding events. Thus, SPR could potentially serve as an attractive technique for rapid, sensitive, reliable, and label-free cancer diagnoses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1520-6882
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8441-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Simultaneous and label-free determination of wild-type and mutant p53 at a single surface plasmon resonance chip preimmobilized with consensus DNA and monoclonal antibody.
pubmed:affiliation
College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, People's Republic of China 410083.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't