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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2009-9-23
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pubmed:abstractText |
Many cellular multi-protein complexes are naturally present in cells at low abundance. Baculovirus expression offers one approach to produce milligram quantities of correctly folded and processed eukaryotic protein complexes. However, current strategies suffer from the need to produce large transfer vectors, and the use of repeated promoter sequences in baculovirus, which itself produces proteins that promote homologous recombination. One possible solution to these problems is to construct baculovirus genomes that express each protein in a complex from a separate locus within the viral DNA. However current methods for selecting such recombinant genomes are too inefficient to routinely modify the virus in this way.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2199
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
87
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:19725957-Animals,
pubmed-meshheading:19725957-Baculoviridae,
pubmed-meshheading:19725957-Chaperonin Containing TCP-1,
pubmed-meshheading:19725957-Chaperonins,
pubmed-meshheading:19725957-Gene Expression,
pubmed-meshheading:19725957-Genetic Engineering,
pubmed-meshheading:19725957-Genetic Vectors,
pubmed-meshheading:19725957-Genome, Viral,
pubmed-meshheading:19725957-Mice,
pubmed-meshheading:19725957-Recombinant Proteins,
pubmed-meshheading:19725957-Spodoptera
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pubmed:year |
2009
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pubmed:articleTitle |
Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA.
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pubmed:affiliation |
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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