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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 9
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pubmed:dateCreated |
2009-9-2
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pubmed:abstractText |
The crystal structure of the esterase EstA from the cold-adapted bacterium Pseudoalteromonas sp. 643A was determined in a covalently inhibited form at a resolution of 1.35 A. The enzyme has a typical SGNH hydrolase structure consisting of a single domain containing a five-stranded beta-sheet, with three helices at the convex side and two helices at the concave side of the sheet, and is ornamented with a couple of very short helices at the domain edges. The active site is located in a groove and contains the classic catalytic triad of Ser, His and Asp. In the structure of the crystal soaked in diethyl p-nitrophenyl phosphate (DNP), the catalytic serine is covalently connected to a phosphonate moiety that clearly has only one ethyl group. This is the only example in the Protein Data Bank of a DNP-inhibited enzyme with covalently bound monoethylphosphate.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1744-3091
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
862-5
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pubmed:dateRevised |
2010-9-2
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pubmed:meshHeading |
pubmed-meshheading:19724118-Amino Acid Sequence,
pubmed-meshheading:19724118-Bacterial Proteins,
pubmed-meshheading:19724118-Carboxylic Ester Hydrolases,
pubmed-meshheading:19724118-Crystallography, X-Ray,
pubmed-meshheading:19724118-Enzyme Inhibitors,
pubmed-meshheading:19724118-Molecular Sequence Data,
pubmed-meshheading:19724118-Organophosphorus Compounds,
pubmed-meshheading:19724118-Palmitoyl-CoA Hydrolase,
pubmed-meshheading:19724118-Phosphonic Acids,
pubmed-meshheading:19724118-Protein Structure, Secondary,
pubmed-meshheading:19724118-Pseudoalteromonas,
pubmed-meshheading:19724118-Sequence Alignment,
pubmed-meshheading:19724118-Static Electricity
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pubmed:year |
2009
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pubmed:articleTitle |
Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.
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pubmed:affiliation |
Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, IL 60439, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural,
Research Support, N.I.H., Intramural
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