Linked Life Data

19722208

Source:http://linkedlifedata.com/resource/pubmed/id/19722208

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2009-9-23
pubmed:abstractText
This paper demonstrates a microfluidic chip for multiple immunoassays on electrospun (ES) nanofibrous membranes made of PVDF. This microchip enables the detection of multiple pairs of protein-protein interactions in one experiment by crossing parallel arrays of microchannels. This chip uses ESPVDF membrane with high specific surface area as the substrate to adsorb protein for immobilized immunoassays so that increased levels of protein adsorption may lower the LOD in immunoassays. We employ a simple and effective method for chip assembly by scotch tape to incorporate the ESPVDF membrane into the microchip. We believe that the present work will facilitate the development of methods for the on-site diagnosis of diseases whose detection is based on antigen-antibody recognition, and improve the efficiencies of certain experiments that need to identify tens to hundreds of protein-protein interactions with relatively low cost in the future.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1522-2683
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3269-75
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Incorporation of electrospun nanofibrous PVDF membranes into a microfluidic chip assembled by PDMS and scotch tape for immunoassays.
pubmed:affiliation
CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, P. R. China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't