Source:http://linkedlifedata.com/resource/pubmed/id/19718793
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2009-8-31
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| pubmed:abstractText |
In vivo molecularly targeted fluorescence imaging of tumors has been proposed as a strategy for improving cancer detection and management. Activatable fluorophores, which increased their fluorescence by 10-fold after binding tumor cells, result in much higher target to background ratios than conventional fluorophores. We developed an in vivo targeted activatable optical imaging probe based on a fluorophore-quencher pair, bound to a targeting moiety. With this system, fluorescence is quenched by the fluorophore-quencher interaction outside cancer cells, but is activated within the target cells by dissociation of the fluorophore-quencher pair. We selected the TAMRA (fluorophore)-QSY7 (quencher) pair and conjugated it to either avidin (targeting the D-galactose receptor) or trastuzumab (a monoclonal antibody against the human epithelial growth factor receptor type2 (HER2/neu)) and evaluated their performance in mouse models of cancer. Two probes, TAMRA-QSY7 conjugated avidin (Av-TM-Q7) and trastuzumab (Traz-TM-Q7) were synthesized. Both demonstrated better than similar self-quenching probes. In vitro fluorescence microscopic studies of SHIN3 and NIH/3T3/HER2+ cells demonstrated that Av-TM-Q7 and Traz-TM-Q7 produced high intracellular fluorescent signal. In vivo imaging with Av-TM-Q7 and Traz-TM-Q7 in mice enabled the detection of small tumors. This molecular imaging probe, based on a fluorophore-quencher pair conjugated to a targeting ligand, successfully detected tumors in vivo due to its high activation ratio and low background signal. Thus, these activatable probes, based on the fluorophore-quencher system, hold promise clinically for "see and treat" strategies of cancer management.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-carboxytetramethylrhodamine...,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, Humanized,
http://linkedlifedata.com/resource/pubmed/chemical/Avidin,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Rhodamines,
http://linkedlifedata.com/resource/pubmed/chemical/enhanced green fluorescent protein,
http://linkedlifedata.com/resource/pubmed/chemical/galactose receptor,
http://linkedlifedata.com/resource/pubmed/chemical/herceptin-rhodamine green conjugate
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1543-8384
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
6
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
386-95
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:19718793-Animals,
pubmed-meshheading:19718793-Antibodies, Monoclonal,
pubmed-meshheading:19718793-Antibodies, Monoclonal, Humanized,
pubmed-meshheading:19718793-Avidin,
pubmed-meshheading:19718793-BALB 3T3 Cells,
pubmed-meshheading:19718793-Female,
pubmed-meshheading:19718793-Fluorescent Dyes,
pubmed-meshheading:19718793-Green Fluorescent Proteins,
pubmed-meshheading:19718793-Humans,
pubmed-meshheading:19718793-Lung Neoplasms,
pubmed-meshheading:19718793-Mice,
pubmed-meshheading:19718793-Mice, Nude,
pubmed-meshheading:19718793-Microscopy, Fluorescence,
pubmed-meshheading:19718793-NIH 3T3 Cells,
pubmed-meshheading:19718793-Receptor, erbB-2,
pubmed-meshheading:19718793-Receptors, Cell Surface,
pubmed-meshheading:19718793-Rhodamines,
pubmed-meshheading:19718793-Transfection
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| pubmed:articleTitle |
Fluorophore-quencher based activatable targeted optical probes for detecting in vivo cancer metastases.
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| pubmed:affiliation |
Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 10 Center Drive, Bethesda, Maryland 20892-1088, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
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