Linked Life Data

19718715

Source:http://linkedlifedata.com/resource/pubmed/id/19718715

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-9-29
pubmed:abstractText
Caveolin-1 (CAV-1) has been reported to play an important role in the development of a variety of human cancers. CAV-1 expression is revealed to be reduced or absent in the malignant tumor cells of small cell lung cancers (SCLC). This study was performed to investigate the influences of the stable expression of CAV-1 on the metastasis and proliferation of SCLC in vitro. The wild-type CAV-1 gene was successfully transfected into the NCI-H446 cells and was stably expressed in the NCI-H446 cells. The effects of CAV-1 on the morphology, proliferation, and metastasis potential for NCI-H446 cell were evaluated by crystal violet staining, MTT analysis, transwell assay, and scratch wound assay, respectively. Western blot and gelatin zymography were used to examine the expression changes of the metastasis-related MMP-3 and E-cadherin. Stable expression of CVA-1 was observed in the H446-CAV-1 cells, which enlarged the cell shape with filopodia. The proliferation of H446-CAV-1 was inhibited, while its migration and invasion abilities were promoted in vitro. The re-expression of CAV-1 reduced the expression of E-cadherin, while it increased the protein expression and enzyme activity of MMP-3. Taken together, the cellular proliferation of the NCI-H446 could be inhibited by the re-expression of CAV-1. CAV-1 might increase the cell metastasis potential through the interaction with E-cadherin and MMP-3 genes. These in vitro findings confirm the involvement of CAV-1 in the proliferation and metastasis of SCLC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1932-8494
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
292
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1584-92
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Caveolin-1 is an important factor for the metastasis and proliferation of human small cell lung cancer NCI-H446 cell.
pubmed:affiliation
Department of Histology and Embryology, Dalian Medical University, Dalian 116044, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't