Source:http://linkedlifedata.com/resource/pubmed/id/19712029
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2010-1-27
|
| pubmed:abstractText |
The efficacy of Schwann-cell cultivation can be enhanced by in vitro predegeneration of the harvested cells compared to immediate culture. The aim of this study was to improve Schwann-cell culture efficacy by comparing three different durations of predegeneration. The sciatic and median nerves of 6-8-week-old Lewis rats were harvested and subjected to either 2-day, 7-day, or 14-day predegeneration in Dulbecco's Modified Eagle's Medium supplemented with 10% fetal calf serum and 1% Penicillin/Streptomycin. Afterward, tissue was enzymatically dissociated and placed in a modified melanocyte growth medium. The cell count was determined immediately after dissociation while the cell purity was determined one subculture/trypsinization cycle later after cell attachment to the culture plate by means of optical microscopy and immunocytochemistry. Particular attention was then paid to the Schwann-cell-to-fibroblast relation. The cumulative cell count in the culture was 5.8 x 10(5) for 2-day, 1.12 x 10(6) for 7-day, and 1.48 x 10(6) for 14-day predegeneration. The culture purity was approximately equal for 2- and 7-day predegeneration (88% Schwann cells, 12% fibroblasts after 2 days; 85% Schwann cells, 15% fibroblasts after 7 days). After 14 days, however, cell cultures were significantly debased by fibroblast proliferation (57% Schwann cells, 43% fibroblasts). In vitro predegeneration is a particularly suitable procedural method to increase the cultural Schwann-cell yield. The number of cultivated rat Schwann cells is doubled by 7-day in vitro predegeneration in comparison to 2-day predegeneration. After 14-day predegeneration, however, the culture is significantly debased by fibroblasts. Therefore, 7-day in vitro predegeneration is an advisable predegeneration period.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/S-100 calcium-binding protein beta...,
http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TNFRSF16 protein, rat
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
1557-9042
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
27
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
197-203
|
| pubmed:meshHeading |
pubmed-meshheading:19712029-Animals,
pubmed-meshheading:19712029-Biological Markers,
pubmed-meshheading:19712029-Cell Count,
pubmed-meshheading:19712029-Cell Culture Techniques,
pubmed-meshheading:19712029-Cell Proliferation,
pubmed-meshheading:19712029-Cells, Cultured,
pubmed-meshheading:19712029-Fibroblasts,
pubmed-meshheading:19712029-Immunohistochemistry,
pubmed-meshheading:19712029-Median Nerve,
pubmed-meshheading:19712029-Nerve Growth Factors,
pubmed-meshheading:19712029-Organ Culture Techniques,
pubmed-meshheading:19712029-Peripheral Nerves,
pubmed-meshheading:19712029-Rats,
pubmed-meshheading:19712029-Rats, Inbred Lew,
pubmed-meshheading:19712029-Receptors, Nerve Growth Factor,
pubmed-meshheading:19712029-S100 Proteins,
pubmed-meshheading:19712029-Schwann Cells,
pubmed-meshheading:19712029-Sciatic Nerve,
pubmed-meshheading:19712029-Spinal Cord Injuries,
pubmed-meshheading:19712029-Time Factors,
pubmed-meshheading:19712029-Tissue Transplantation,
pubmed-meshheading:19712029-Wallerian Degeneration
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Efficacy of various durations of in vitro predegeneration on the cell count and purity of rat Schwann-cell cultures.
|
| pubmed:affiliation |
Department of Hand, Plastic, Reconstructive and Burn Surgery, BG-Trauma Center, Eberhard Karls University, Tüebingen, Germany.
|
| pubmed:publicationType |
Journal Article
|