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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
34
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pubmed:dateCreated |
2009-8-27
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pubmed:abstractText |
We designed phosphorothioate-modified DNA probes linked to superparamagnetic iron oxide nanoparticles (SPION) for in vivo magnetic resonance imaging (MRI) of fosB and Delta fosB mRNA after amphetamine (AMPH) exposure in mice. Specificity of both the fosB and Delta fosB probes was verified by in vitro reverse transcriptase-PCR amplification to a single fragment of total cDNA obtained from acutely AMPH-exposed mouse brains. We confirmed time-dependent uptake and retention profiles of both probes in neurons of GAD67-green fluorescent protein knock-in mice. MRI signal of SPION-labeled fosB probe delivered via intracerebroventricular route was elevated in both acutely and chronically AMPH-exposed mice; the signal was suppressed by dopaminergic receptor antagonist pretreatment. SPION-labeled Delta fosB probe signal elevation occurred only in chronically AMPH-exposed mice. The in vivo target specificity of these probes permits reliable MRI visualization of AMPH-induced differential elevations of fosB and Delta fosB mRNA in living brains.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/P30 NS045776-06A17807,
http://linkedlifedata.com/resource/pubmed/grant/P30NS045776,
http://linkedlifedata.com/resource/pubmed/grant/P41 RR014075-10,
http://linkedlifedata.com/resource/pubmed/grant/P41RR014075,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA020796-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 DA026108-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 EB002066-19,
http://linkedlifedata.com/resource/pubmed/grant/R01 NS045845-04,
http://linkedlifedata.com/resource/pubmed/grant/R01DA020796,
http://linkedlifedata.com/resource/pubmed/grant/R01DA026108,
http://linkedlifedata.com/resource/pubmed/grant/R01EB002066,
http://linkedlifedata.com/resource/pubmed/grant/R01NS045845,
http://linkedlifedata.com/resource/pubmed/grant/R03EB008134,
http://linkedlifedata.com/resource/pubmed/grant/R21 DA024235-02,
http://linkedlifedata.com/resource/pubmed/grant/R21 NS057556-01A1S1,
http://linkedlifedata.com/resource/pubmed/grant/R21 NS057556-02,
http://linkedlifedata.com/resource/pubmed/grant/R21DA024235,
http://linkedlifedata.com/resource/pubmed/grant/R21NS057556
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-10499584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-10611372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-11403691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-11572966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-11579619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-11689160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-12596507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-12890524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-1374894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-14534245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-14574680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-15010207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-15519672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-15564575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-15659295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-16170302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-16242410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-16284251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-1631058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-17026527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-17234603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-17324382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-17478745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-17532882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-17786200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-18029447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-18184321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-18632938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-18657800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-1900040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-19295156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-2062986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-7944289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-7946359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-8524024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-8603091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-8667013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-8706134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-9204924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-9294222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-9668659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19710318-9952411
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amphetamine,
http://linkedlifedata.com/resource/pubmed/chemical/Central Nervous System Stimulants,
http://linkedlifedata.com/resource/pubmed/chemical/Contrast Media,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Fosb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Decarboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorothioate Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/glutamate decarboxylase 1
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1529-2401
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
26
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