Linked Life Data

19709818

Source:http://linkedlifedata.com/resource/pubmed/id/19709818

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2010-2-9
pubmed:abstractText
Interaction of Streptococcus suis with primary porcine alveolar macrophages was studied using transcriptomics. Transcriptional response of macrophages to two different S. suis strains was studied: wild-type S10 that is resistant to phagocytosis, and its non-encapsulated mutant that is phagocytosed efficiently. The macrophages' transcriptional response was observed only after 60 min of incubation. Eleven genes were expressed significantly different between macrophages infected with streptococci and control mock-infected macrophages. These genes include IL-1-beta, MIP-2-alpha and TNF-alpha. When gene expression was studied as a function of time, transcriptional changes occurred in all macrophages independent of streptococci. The fold induction of induced genes however, was much stronger in macrophages incubated with the non-encapsulated S. suis strain that was phagocytosed. The genes that were higher induced due to S. suis suggest an innate immune response is induced in macrophages. Pathway analysis revealed that genes that are part of the putative MAP-kinase signal transduction system are over-represented among the regulated genes. Using an immortalized alveolar macrophage cell line it was shown that macrophages respond to interaction with S. suis by the translocation of NF-kappaB to the nucleus, independent of phagocytosis. This translocation subsequently induced expression of innate immune genes. This strongly suggests besides the MAP-kinase signaling pathway, NF-kappaB signaling is also induced upon interaction with S. suis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1873-2542
pubmed:author
pubmed:copyrightInfo
Copyright 2009 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
24
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-67
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Involvement of NF-kappaB and MAP-kinases in the transcriptional response of alveolar macrophages to Streptococcus suis.
pubmed:affiliation
Central Veterinary Institute of Wageningen University and Research Center, Edelhertweg 15, 8219 PH Lelystad, The Netherlands. astrid.degreeff@wur.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't