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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1990-5-31
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pubmed:abstractText |
Active accumulation of neurotransmitters by synaptic vesicles is an essential component of the synaptic transmission cycle. Isolated vesicles show energy-dependent uptake of several transmitters by processes which are apparently mediated by a proton electrochemical potential across the vesicle membrane. Although this energy gradient is probably generated by a proton ATPase, the functional separation of ATP cleavage and transmitter uptake activity has only been shown clearly for monoamine transport. We report here that the light-driven proton pump, bacteriorhodopsin, can replace the endogenous proton ATPase in proteoliposomes reconstituted from vesicular detergent extracts. The system shows light-dependent uptake of glutamate with properties very similar to those observed in intact vesicles, e.g. chloride dependence or stimulation by NH4+. Our experiments show that the proton pump and the glutamate transporter are separate entities and provide a powerful tool for further characterization of the glutamate carrier.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-1688846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-1691873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-229771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2440881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2566998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2577130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2856886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2872676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2878429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2883008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2892215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2902091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2903047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2906725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2937444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-2964510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-3072701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-4418026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-5834239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-6129842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-6311813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-6928624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-6992165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1970294-7387697
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1465-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1970294-Amino Acid Transport System X-AG,
pubmed-meshheading:1970294-Animals,
pubmed-meshheading:1970294-Bacteriorhodopsins,
pubmed-meshheading:1970294-Biological Transport, Active,
pubmed-meshheading:1970294-Glutamates,
pubmed-meshheading:1970294-Glutamic Acid,
pubmed-meshheading:1970294-Glycoproteins,
pubmed-meshheading:1970294-Liposomes,
pubmed-meshheading:1970294-Rats,
pubmed-meshheading:1970294-Synaptic Vesicles,
pubmed-meshheading:1970294-Tritium
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pubmed:year |
1990
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pubmed:articleTitle |
Bacteriorhodopsin drives the glutamate transporter of synaptic vesicles after co-reconstitution.
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pubmed:affiliation |
Department of Neurochemistry, Max-Planck-Institute for Psychiatry, Martinsried, FRG.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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