Linked Life Data

19699244

Source:http://linkedlifedata.com/resource/pubmed/id/19699244

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-11-9
pubmed:abstractText
Functionalized biodegradable nanoparticles (NPs) provide reactive groups and large surface area for grafting recombinant human bone morphogenetic protein-2 (rhBMP-2) to reduce protein diffusion and maintain sufficient concentration for recruitment and differentiation of osteoprogenitor cells. The objective of this work was to investigate release characteristics and osteogenic activity of rhBMP-2, grafted to biodegradable NPs based on succinimide-terminated poly(lactide fumarate) (PLAF-NHS) and poly(lactide-co-glycolide fumarate) (PLGF-NHS) macromers. The release of rhBMP-2 from the NPs, measured by enzyme-linked immunosorbent assay, was linear with time in the first two weeks, and 24.70+/-1.30% and 48.7+/-0.7% of the protein grafted to PLGF-NHS and PLAF-NHS NPs, respectively, was released in the enzymatically active conformation after complete degradation/erosion of the NPs. After 14 days of incubation with bone marrow stromal (BMS) cells, rhBMP-2 grafted to PLAF-NHS and PLGF-NHS NPs was as effective in inducing mineralization as the native rhBMP-2 that was directly added to the cell culture media. At any incubation time, rhBMP-2 grafted to PLAF had the highest expression of osteopontin (OP) and osteocalcin (OC), followed by rhBMP-2 grafted to PLGF and rhBMP-2 directly added to media. Higher OP and OC expression for BMP-gPLAF and BMP-gPLGF groups may be related to other factors in the cascade of osteogenesis, such as differentiation of BMS cells to the vasculogenic lineage and formation of a vascularized/mineralized matrix.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1873-4995
pubmed:author
pubmed:issnType
Electronic
pubmed:day
3
pubmed:volume
140
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
148-56
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
pubmed-meshheading:19699244-Animals, pubmed-meshheading:19699244-Bone Marrow Cells, pubmed-meshheading:19699244-Bone Morphogenetic Protein 2, pubmed-meshheading:19699244-Calcification, Physiologic, pubmed-meshheading:19699244-Cells, Cultured, pubmed-meshheading:19699244-Chemistry, Pharmaceutical, pubmed-meshheading:19699244-Drug Carriers, pubmed-meshheading:19699244-Drug Compounding, pubmed-meshheading:19699244-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:19699244-Humans, pubmed-meshheading:19699244-Kinetics, pubmed-meshheading:19699244-Male, pubmed-meshheading:19699244-Nanoparticles, pubmed-meshheading:19699244-Osteocalcin, pubmed-meshheading:19699244-Osteogenesis, pubmed-meshheading:19699244-Osteopontin, pubmed-meshheading:19699244-Polyesters, pubmed-meshheading:19699244-Rats, pubmed-meshheading:19699244-Rats, Wistar, pubmed-meshheading:19699244-Recombinant Proteins, pubmed-meshheading:19699244-Solubility, pubmed-meshheading:19699244-Stromal Cells
pubmed:year
2009
pubmed:articleTitle
Release characteristics and osteogenic activity of recombinant human bone morphogenetic protein-2 grafted to novel self-assembled poly(lactide-co-glycolide fumarate) nanoparticles.
pubmed:affiliation
Biomimetic Materials and Tissue Engineering Laboratory, Department of Chemical Engineering, University of South Carolina, Columbia, SC 29208, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural