Linked Life Data

19683811

Source:http://linkedlifedata.com/resource/pubmed/id/19683811

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
31
pubmed:dateCreated
2009-9-11
pubmed:abstractText
We aimed to develop a hybrid scaffold with a porous structure and similar composition as natural bone for the controlled release of bone morphogenetic protein-2 (BMP-2) to enhance bone regeneration. We fabricated a gelatin/nanohydroxypatite (nHAP) scaffold by glutaraldehyde chemical cross-linking a gelatin aqueous solution with nHAP granules at a 5:1 ratio (v/w). Then, fibrin glue (FG) mixed with recombinant human BMP-2 (rhBMP-2) was infused into the gelatin/nHAP scaffold and lyophilized to develop an rhBMP-2-loaded gelatin/nHAP/FG scaffold. On scanning electron microscopy, the composite had a 3-D porous structure. The rhBMP-2 release kinetics from the hybrid scaffold was sustained and slow, and release of rhBMP-2 was complete at 40 days. Immunohistochemistry, azo-coupling and alizarin S-red staining were used to study in vitro differentiation of human bone-marrow mesenchymal cells (hBMSCs). Strong positive staining results confirmed that rhBMP-2 released from the scaffold could improve osteocalcin (OCN) and alkaline phosphatase (ALP) expression and calcium deposition formation. RT-PCR results showed significantly high mRNA expression of ALP and OCN in hBM-MSCs cultured on the gelatin/nHAP/FG scaffold with rhBMP-2. DNA assay demonstrated that the scaffold was noncytotoxic and could promote hBMSC proliferation from the components of the hybrid scaffold, not released rhBMP-2. The hybrid scaffolds were then used to repair critical-size segmental bone defects of rabbit radius. Gross specimen, X-ray, bone histomorphology and bone mineral density assay demonstrated that the rhBMP-2-loaded gelatin/nHAP/FG scaffold had good osteogenic capability and could repair the segmental bone defect completely in 12 weeks.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1878-5905
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6276-85
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:19683811-Animals, pubmed-meshheading:19683811-Bone Morphogenetic Protein 2, pubmed-meshheading:19683811-Bone Morphogenetic Proteins, pubmed-meshheading:19683811-Bone Regeneration, pubmed-meshheading:19683811-Durapatite, pubmed-meshheading:19683811-Female, pubmed-meshheading:19683811-Fibrin, pubmed-meshheading:19683811-Gelatin, pubmed-meshheading:19683811-Humans, pubmed-meshheading:19683811-Kinetics, pubmed-meshheading:19683811-Male, pubmed-meshheading:19683811-Microscopy, Electron, Scanning, pubmed-meshheading:19683811-Rabbits, pubmed-meshheading:19683811-Recombinant Proteins, pubmed-meshheading:19683811-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:19683811-Tissue Engineering, pubmed-meshheading:19683811-Tissue Scaffolds, pubmed-meshheading:19683811-Transforming Growth Factor beta
pubmed:year
2009
pubmed:articleTitle
Segmental bone regeneration using an rhBMP-2-loaded gelatin/nanohydroxyapatite/fibrin scaffold in a rabbit model.
pubmed:affiliation
Department of Orthopedics, Tianjin Hospital, No. 406 Jiefang South Road, Hexi District, Tianjin 300211, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't