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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1991-9-27
|
| pubmed:abstractText |
A method is presented for the preparation of large DNA molecules from protoplasts embedded in agarose blocks of three different cereals--hexaploid bread wheat (Triticum aestivum), barley (Hordeum vulgare) and rye (Secale cereale). Pulse-field gel electrophoresis (PFGE) analysis of these DNA preparations using a contour-clamped homogeneous field (CHEF) apparatus indicated that the size of the DNA molecules was greater than 6 Mb. DNA samples prepared by this method were shown to be useful for restriction analysis using both frequent and rare cutting enzymes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0167-4412
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
881-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1966389-DNA,
pubmed-meshheading:1966389-DNA Restriction Enzymes,
pubmed-meshheading:1966389-Electrophoresis, Agar Gel,
pubmed-meshheading:1966389-Hordeum,
pubmed-meshheading:1966389-Molecular Weight,
pubmed-meshheading:1966389-Plants,
pubmed-meshheading:1966389-Secale cereale,
pubmed-meshheading:1966389-Triticum
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis.
|
| pubmed:affiliation |
Cambridge Laboratory, Colney Lane, Norwich, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|