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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2009-11-12
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pubmed:abstractText |
Transcriptional control of stem cell genes is a critical step in differentiation of embryonic stem cells and in reprogramming of somatic cells into stem cells. Here we report that Lsh, a regulator of repressive chromatin at retrotransposons, also plays an important role in silencing of stem cell-specific genes such as Oct4. We found that CpG methylation is gained during in vitro differentiation of several stem cell-specific genes (in 11 of 12 promoter regions) and thus appears to be a common epigenetic mark. Lsh depletion prevents complete silencing of stem cell gene expression and moreover promotes the maintenance of stem cell characteristics in culture. Lsh is required for establishment of DNA methylation patterns at stem cell genes during differentiation, in part by regulating access of Dnmt3b to its genomic targets. Our results indicate that Lsh is involved in the control of stem cell genes and suggest that Lsh is an important epigenetic modulator during early stem cell differentiation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA (Cytosine-5-)-Methyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA methyltransferase 3B,
http://linkedlifedata.com/resource/pubmed/chemical/Dppa3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Gdf3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Differentiation Factor 3,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nanog protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-3,
http://linkedlifedata.com/resource/pubmed/chemical/Pou5f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tdgf1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/lymphoid specific helicase, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1549-4918
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2691-702
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pubmed:dateRevised |
2010-5-21
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pubmed:meshHeading |
pubmed-meshheading:19650037-Animals,
pubmed-meshheading:19650037-Blotting, Southern,
pubmed-meshheading:19650037-Blotting, Western,
pubmed-meshheading:19650037-Cell Differentiation,
pubmed-meshheading:19650037-Cell Line,
pubmed-meshheading:19650037-Cell Line, Tumor,
pubmed-meshheading:19650037-Chromatin Immunoprecipitation,
pubmed-meshheading:19650037-DNA (Cytosine-5-)-Methyltransferase,
pubmed-meshheading:19650037-DNA Helicases,
pubmed-meshheading:19650037-DNA Methylation,
pubmed-meshheading:19650037-Epidermal Growth Factor,
pubmed-meshheading:19650037-Gene Silencing,
pubmed-meshheading:19650037-Growth Differentiation Factor 3,
pubmed-meshheading:19650037-Homeodomain Proteins,
pubmed-meshheading:19650037-Membrane Glycoproteins,
pubmed-meshheading:19650037-Mice,
pubmed-meshheading:19650037-Neoplasm Proteins,
pubmed-meshheading:19650037-Octamer Transcription Factor-3,
pubmed-meshheading:19650037-Polymerase Chain Reaction,
pubmed-meshheading:19650037-Promoter Regions, Genetic,
pubmed-meshheading:19650037-Protein Binding,
pubmed-meshheading:19650037-Repressor Proteins
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pubmed:year |
2009
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pubmed:articleTitle |
Lsh participates in DNA methylation and silencing of stem cell genes.
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pubmed:affiliation |
Laboratory of Cancer Prevention, SAIC-Frederick, National Cancer Institute, Frederick, MD 21701, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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