Source:http://linkedlifedata.com/resource/pubmed/id/19648268
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2009-8-21
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pubmed:abstractText |
It is known that C3 is required for optimal expansion of T cells during acute viral infections. However, it is not yet determined whether T cell responses to intracellular bacterial infections require C3. Therefore, we have investigated the requirement for C3 to elicit potent T cell responses to Listeria monocytogenes (LM). We show that expansion of Ag-specific CD8 and CD4 T cells during a primary response to LM was markedly reduced in the absence of C3 activity. Further studies indicated that, unlike in an influenza virus infection, the regulation of LM-specific T cell responses by C3 might not involve the downstream effector C5a. Moreover, reduced T cell responses to LM was not linked to defective maturation of dendritic cells or developmental anomalies in the peripheral T cell compartment of C3-deficient mice. Experiments involving adoptive transfer of C3-deficient CD8 T cells into the C3-sufficient environment of wild-type mice showed that these T cells do not have intrinsic proliferative defects, and a paracrine source of C3 will suffice for clonal expansion of CD8 T cells in vivo. However, stimulation of purified C3-deficient CD8 T cells by plastic-immobilized anti-CD3 showed that C3 promotes T cell proliferation directly, independent of its effects on APC. On the basis of these findings, we propose that diminished T cell responses to LM in C3-deficient mice might be at least in part due to lack of direct effects of C3 on T cells. These studies have furthered our understanding of C3-mediated regulation of T cell immunity to intracellular pathogens.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/AI068841,
http://linkedlifedata.com/resource/pubmed/grant/AI48785,
http://linkedlifedata.com/resource/pubmed/grant/AI59804,
http://linkedlifedata.com/resource/pubmed/grant/AI68730,
http://linkedlifedata.com/resource/pubmed/grant/GM062134,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI048785-07,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI048785-09,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI059804-05,
http://linkedlifedata.com/resource/pubmed/grant/R21 AI068841-02
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1550-6606
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
183
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2921-31
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pubmed:dateRevised |
2010-12-3
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pubmed:meshHeading |
pubmed-meshheading:19648268-Animals,
pubmed-meshheading:19648268-CD4-Positive T-Lymphocytes,
pubmed-meshheading:19648268-CD8-Positive T-Lymphocytes,
pubmed-meshheading:19648268-Cell Proliferation,
pubmed-meshheading:19648268-Complement C3,
pubmed-meshheading:19648268-Listeria monocytogenes,
pubmed-meshheading:19648268-Listeriosis,
pubmed-meshheading:19648268-Lymphocyte Activation,
pubmed-meshheading:19648268-Mice,
pubmed-meshheading:19648268-Mice, Inbred C57BL,
pubmed-meshheading:19648268-Mice, Knockout,
pubmed-meshheading:19648268-Mice, Transgenic,
pubmed-meshheading:19648268-Receptor, Anaphylatoxin C5a
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pubmed:year |
2009
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pubmed:articleTitle |
C3 promotes expansion of CD8+ and CD4+ T cells in a Listeria monocytogenes infection.
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pubmed:affiliation |
Department of Pathobiological Sciences, University of Wisconsin, Madison, WI 53706, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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