Source:http://linkedlifedata.com/resource/pubmed/id/19616865
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2009-8-24
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pubmed:abstractText |
Non-thermal sterilization and microbial inactivation processes are currently receiving much attention in food and pharmaceutical industries. In particular, since supercritical carbon dioxide (SC-CO2) treatment, which is conducted at relatively low temperatures, is considered to be a promising alternative method to replace thermal sterilization processes that cannot be safely used in foods and bioactive materials. Although SC-CO2 has been applied to many microorganisms, the inactivation of microbial cells by SC-CO2 has only been evaluated by using a conventional viable cell count such as a plating method, by which it is not possible to systematically elucidate the microbial cell inactivation process. Therefore, in this study the physiological status of SC-CO2 treated Salmonella enterica serotype Typhimurium was analyzed by using GC-MS analysis of fatty acids with principal component analysis and two-dimensional electrophoresis for protein profiling. From the results of these systemic analyses, it was revealed that SC-CO2 caused significant alterations to the profiles of fatty acids and proteins of the cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1879-3460
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
134
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
190-5
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pubmed:meshHeading |
pubmed-meshheading:19616865-Bacterial Proteins,
pubmed-meshheading:19616865-Carbon Dioxide,
pubmed-meshheading:19616865-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:19616865-Fatty Acids,
pubmed-meshheading:19616865-Food Microbiology,
pubmed-meshheading:19616865-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:19616865-Microbial Viability,
pubmed-meshheading:19616865-Principal Component Analysis,
pubmed-meshheading:19616865-Protein Array Analysis,
pubmed-meshheading:19616865-Proteome,
pubmed-meshheading:19616865-Salmonella typhimurium,
pubmed-meshheading:19616865-Sterilization
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pubmed:year |
2009
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pubmed:articleTitle |
Fatty acid profiling and proteomic analysis of Salmonella enterica serotype Typhimurium inactivated with supercritical carbon dioxide.
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pubmed:affiliation |
School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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