Linked Life Data

1960704

Source:http://linkedlifedata.com/resource/pubmed/id/1960704

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-1-6
pubmed:abstractText
Major rat sperm autoantigens of 86, 63, 43, 28 and 20 kDa are recognized by post-vasectomy and hyperimmunization antisera from the Lewis rat (Handley et al., Biol. Reprod. 39 (1988) 1239-1250). In the present study, affinity purified monospecific isoantibodies to each autoantigen were produced by elution from antigens which had been separated by SDS-PAGE and transferred to nitrocellulose. Western blot analysis confirmed a singular specificity for the 63, 28 and 20 kDa antisera and demonstrated some cross reactivity between the 86 kDa and the 43 kDa antisera. The polyclonal antiserum from which the monospecific antisera were produced stained the entire spermatozoon, while monospecific antibodies bound only to the sperm tail, staining the proximal portion (43 and 28 kDa), a distal domain (63 kDa), or the entire tail (86 kDa). Immunohistochemically stained sections of normal rat testes revealed that the 63, 43 and 28 kDa autoantigens were synchronously expressed in the cytoplasm of spermatids in the apical portions of seminiferous tubules during stages II-VIII in the cycle of the seminiferous epithelium. The 86 kDa autoantigen showed little or no staining in testis sections, implying that this autoantigen appeared on mature sperm following spermiation. These and other data suggest that a highly polymeric structure, possibly within the outer dense fibers of the tail, is a dominant sperm autoimmunogen following vasectomy of the Lewis rat.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0165-0378
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
205-20
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Localization of post-vasectomy sperm autoantigens in the Lewis rat.
pubmed:affiliation
Department of Anatomy and Cell Biology, University of Virginia, Charlottesville 22908.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.