Linked Life Data

19606849

Source:http://linkedlifedata.com/resource/pubmed/id/19606849

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-8-25
pubmed:abstractText
In this article, we report a novel nanoparticle-enhanced biophysical technique that differentiates multipotent marrow stromal cells (MSCs) toward osteoblasts. We show that a brief (10 min) daily nanoparticle-facilitated exposure of MSCs to nanosecond pulse laser-induced photoacoustic (PA) stimulation enhances their differentiation toward osteoblasts. To observe osteodifferentiation under PA stimulation, tissue culture plates were seeded with MSCs without the osteogenic culture supplements (OS, 0.01 M beta-glycerophosphate, 50 mg/L ascorbic acid, 10(-8) M dexamethasone) in the presence and absence of single-walled carbon nanotubes (SWNTs) and gold nanoparticles (GNPs). The alkaline phosphatase activity, calcium content, and osteopontin secretion were monitored as indicators of MSCs' differentiation toward osteoblasts. The PA stimulated groups show up to 612% increase in calcium content compared to the controls cultured with osteogenic supplements (without PA stimulation) after 16 days. Among the PA stimulated groups, at day 16, MSCs incubated with SWNTs at 10 microg/mL concentrations showed up to 97% greater calcium content than those that did not contain SWNTs. The results demonstrated that PA stimulation not only promotes osteogenesis but also is synergistically enhanced by the presence of nanoparticles and, thus, has major implications for bone regeneration applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1936-086X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
25
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2065-72
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
The effect of nanoparticle-enhanced photoacoustic stimulation on multipotent marrow stromal cells.
pubmed:affiliation
Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-2300, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't