Source:http://linkedlifedata.com/resource/pubmed/id/19585113
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2010-1-12
|
| pubmed:abstractText |
Analysis of the alpha-lipomycin biosynthesis gene cluster of Streptomyces aureofaciens Tü117 led to the identification of five putative regulatory genes, which are congregated into a subcluster. Analysis of the lipReg1-4 and lipX1 showed that they encode components of two-component signal transduction systems (LipReg1 and LipReg2), multiple antibiotics resistance-type regulator (LipReg3), large ATP-binding regulators of the LuxR family-type regulator (LipReg4), and small ribonuclease (LipRegX1), respectively. A combination of targeted gene disruptions, complementation experiments, lipomycin production studies, and gene expression analysis via RT-PCR suggests that all regulatory lip genes are involved in alpha-lipomycin production. On the basis of the obtained data, we propose that LipReg2 controls the activity of LipReg1, which in its turn govern the expression of the alpha-lipomycin pathway-specific regulatory gene lipReg4. The ribonuclease gene lipX1 and the transporter regulator lipReg3 appear to work independently of genes lipReg1, lipReg2, and lipReg4.
|
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
|
| pubmed:issn |
1432-0614
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| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
85
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1069-79
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| pubmed:meshHeading |
pubmed-meshheading:19585113-Anti-Bacterial Agents,
pubmed-meshheading:19585113-Chromosome Mapping,
pubmed-meshheading:19585113-Cloning, Molecular,
pubmed-meshheading:19585113-Escherichia coli,
pubmed-meshheading:19585113-Gene Expression Regulation, Bacterial,
pubmed-meshheading:19585113-Gene Regulatory Networks,
pubmed-meshheading:19585113-Genes, Regulator,
pubmed-meshheading:19585113-Genetic Complementation Test,
pubmed-meshheading:19585113-Glycosides,
pubmed-meshheading:19585113-Microbial Sensitivity Tests,
pubmed-meshheading:19585113-Models, Molecular,
pubmed-meshheading:19585113-Multigene Family,
pubmed-meshheading:19585113-Mutagenesis,
pubmed-meshheading:19585113-Polyenes,
pubmed-meshheading:19585113-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19585113-Sequence Analysis, DNA,
pubmed-meshheading:19585113-Streptomyces aureofaciens
|
| pubmed:year |
2010
|
| pubmed:articleTitle |
Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tü117.
|
| pubmed:affiliation |
Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, L'viv, Ukraine.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|