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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1991-12-31
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pubmed:abstractText |
The sequence selectivity of DNase 1 cleavage has been investigated by site-directed mutagenesis of a chemically synthesised gene. Two key DNA binding residues have been conservatively altered (Y76F and R41K) or have had their side-chains truncated (Y76A and R41A) and the effect on the cleavage of tyr T promoter DNA has been noted. It would appear from these studies that DNase 1 is not sensitive to minor groove width via these DNA-contacting residues, and it is suggested that DNA helical stiffness is a controlling parameter in determining DNase 1 sequence selectivity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-15406373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-2179873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-2254338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-2594767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-2600970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-2989796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-3352748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-3553960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-3627268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-3670410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-6327070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-6350894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1956772-6390428
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
19
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pubmed:geneSymbol |
tyrT
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6129-32
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1956772-Animals,
pubmed-meshheading:1956772-Base Sequence,
pubmed-meshheading:1956772-Cattle,
pubmed-meshheading:1956772-DNA,
pubmed-meshheading:1956772-DNA-Binding Proteins,
pubmed-meshheading:1956772-Deoxyribonuclease I,
pubmed-meshheading:1956772-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1956772-Molecular Sequence Data,
pubmed-meshheading:1956772-Mutagenesis,
pubmed-meshheading:1956772-Pancreas,
pubmed-meshheading:1956772-Promoter Regions, Genetic,
pubmed-meshheading:1956772-Substrate Specificity
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pubmed:year |
1991
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pubmed:articleTitle |
Mutagenesis of the DNA binding residues in bovine pancreatic DNase 1: an investigation into the mechanism of sequence discrimination by a sequence selective nuclease.
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pubmed:affiliation |
Department of Biochemistry (SERC Molecular recognition Initiative Centre), University of Southampton, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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