Linked Life Data

19542433

Source:http://linkedlifedata.com/resource/pubmed/id/19542433

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-6-22
pubmed:abstractText
Mast cell degranulation is pivotal to allergic diseases; investigating novel pathways triggering mast cell degranulation would undoubtedly have important therapeutic potential. FcepsilonRI-mediated degranulation has contradictorily been shown to require SphK1 or SphK2, depending on the reports. We investigated the in vitro and in vivo specific role(s) of SphK1 and SphK2 in FcepsilonRI-mediated responses, using specific small interfering RNA-gene silencing. The small interfering RNA-knockdown of SphK1 in mast cells inhibited several signaling mechanisms and effector functions, triggered by FcepsilonRI stimulation including: Ca(2+) signals, NFkappaB activation, degranulation, cytokine/chemokine, and eicosanoid production, whereas silencing SphK2 had no effect at all. Moreover, silencing SPHK1 in vivo, in different strains of mice, strongly inhibited mast cell-mediated anaphylaxis, including inhibition of vascular permeability, tissue mast cell degranulation, changes in temperature, and serum histamine and cytokine levels, whereas silencing SPHK2 had no effect and the mice developed anaphylaxis. Our data differ from a recent report using SPHK1(-/-) and SPHK2(-/-) mice, which showed that SphK2 was required for FcepsilonRI-mediated mast cell responses. We performed experiments in mast cells derived from SPHK1(-/-) and SPHK2(-/-) mice and show that the calcium response and degranulation, triggered by FcepsilonRI-cross-linking, is not different from that triggered in wild-type cells. Moreover, IgE-mediated anaphylaxis in the knockout mice showed similar levels in temperature changes and serum histamine to that from wild-type mice, indicating that there was no protection from anaphylaxis for either knockout mice. Thus, our data strongly suggest a previously unrecognized compensatory mechanism in the knockout mice, and establishes a role for SphK1 in IgE-mediated mast cell responses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1550-6606
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
183
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
221-7
pubmed:meshHeading
pubmed-meshheading:19542433-Anaphylaxis, pubmed-meshheading:19542433-Animals, pubmed-meshheading:19542433-Cell Degranulation, pubmed-meshheading:19542433-Cells, Cultured, pubmed-meshheading:19542433-Cross-Linking Reagents, pubmed-meshheading:19542433-Gene Silencing, pubmed-meshheading:19542433-Isoenzymes, pubmed-meshheading:19542433-Male, pubmed-meshheading:19542433-Mast Cells, pubmed-meshheading:19542433-Mice, pubmed-meshheading:19542433-Mice, Inbred BALB C, pubmed-meshheading:19542433-Mice, Inbred C57BL, pubmed-meshheading:19542433-Mice, Knockout, pubmed-meshheading:19542433-Phosphotransferases (Alcohol Group Acceptor), pubmed-meshheading:19542433-Protein Transport, pubmed-meshheading:19542433-RNA Interference, pubmed-meshheading:19542433-Receptors, IgE, pubmed-meshheading:19542433-Signal Transduction
pubmed:year
2009
pubmed:articleTitle
Sphingosine kinase 1 is pivotal for Fc epsilon RI-mediated mast cell signaling and functional responses in vitro and in vivo.
pubmed:affiliation
Glasgow Biomedical Research Centre, University of Glasgow, Scotland, United Kingdom.
pubmed:publicationType
Journal Article, Comparative Study