Linked Life Data

19530680

Source:http://linkedlifedata.com/resource/pubmed/id/19530680

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
2009-7-1
pubmed:abstractText
A water-soluble glucan, namely, PD3, was isolated from the fruiting body of Dictyophora indusiata as the method reported previously. Its chemical structure was characterized by GC, FTIR, and (13)C NMR. The results indicated that PD3 is a (1-->3)-beta-D-glucan, with (1-->6)-beta-glucopyranoside side branches. The chain conformation and morphology of PD3 in aqueous solution were investigated by viscometry, rheometer, and laser light scattering (LLS) measurements, atomic force microscopy (AFM), and transmission electron microscopy (TEM), respectively. The weight-average molecular mass (M(w)), radius of gyration (R(g)), hydrodynamic radius (R(h)), and intrinsic viscosity ([eta]) of PD3 in water were determined to be 5.1 x 10(5), 141 nm, 44 nm, and 1440 cm(3) g(-1), respectively, by LLS and viscometry. The structural parameter rho (Rg/Rh) of PD3 was calculated to be 3.4, and the [eta] dependence of C(NaOH) of PD3 is similar to that of triple helical polysaccharides Scleroglucan and Lentinan, suggesting that PD3 exists as a triple helical chain in water. This conclusion was further proved by rheological measurement and AFM observation. Interestingly, the [eta] of PD3 dramatically decreased in a narrow range concentration of NaOH between 0.18 and 0.22 M, higher than that of Scleroglucan and Lentinan (both less than 0.1 M), indicating the helix-coil conformation transition of PD3 is more difficult than that of Scleroglucan and Lentinan. Moreover, with the increase of concentration, PD3 trends to self-assemble to fibrous aggregates in aqueous solution as measured by TEM.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1520-5118
pubmed:author
pubmed:issnType
Electronic
pubmed:day
8
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5918-24
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Structural characterization, chain conformation, and morphology of a beta-(1-->3)-D-glucan isolated from the fruiting body of Dictyophora indusiata.
pubmed:affiliation
School of Medicine and Pharmaceautics, Jiangnan University, Wuxi, Jiangsu Province 214122, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't