Linked Life Data

19530313

Source:http://linkedlifedata.com/resource/pubmed/id/19530313

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-6-15
pubmed:abstractText
Acinetobacter baumannii is a nosocomial pathogen responsible for outbreaks of infection worldwide. The factors associated with its ability to colonize/infect human hosts are largely unknown. Adherence to host cells is the first step in colonization/infection, which can be followed by biofilm formation. A. baumannii ATCC19606(T) biofilm formation on abiotic surfaces depends on expression of the CsuA/BABCDE chaperonee-usher pili assembly system. The present study focused on the involvement of CsuA/BABCDE-dependent pili in the interactions between A. baumannii 19606(T) and human bronchial epithelial cells and sheep erythrocytes. Light microscopy analysis revealed that CsuE-mutant #144 adhered to more bronchial epithelial cells than the parental strain. Similar amounts of interleukin (IL)-6 and IL-8 were produced by bronchial epithelial cells in response to these two bacterial strains. Scanning electron microscopy revealed the presence of two types of surface extensions on ATCC19606(T), i.e., short (29 nm; 5-140 nm) pili and long (260 nm; 143-1008 nm) extensions. The latter were not observed on the CsuE-mutant and therefore are likely the previously described CsuA/BABCDE-encoded extensions. We conclude that CsuA/BABCDE-dependent pili are not involved in adherence of A. baumannii ATCC19606(T) to bronchial epithelial cells. The structure of the short pili and their possible role in adherence to human cells requires further investigation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1769-7123
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
160
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-8
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
CsuA/BABCDE-dependent pili are not involved in the adherence of Acinetobacter baumannii ATCC19606(T) to human airway epithelial cells and their inflammatory response.
pubmed:affiliation
Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands. a.de_breij@lumc.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural