|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
Pt 7
|
|
pubmed:dateCreated |
2009-7-6
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
A taxonomic study was carried out on a strain, designated W3-3A(T), which was isolated from a pyrene-degrading consortium, enriched from sediment of the Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W3-3A(T) belonged to the genus Bowmanella, with the highest sequence similarity (99.0 %) with Bowmanella denitrificans BD1(T), whereas sequence similarities with other species were less than 93 %. The nucleotide sequence similarity of both gyrB and rpoD genes of strain W3-3A(T) and B. denitrificans BD1(T) was 81.1 %. However, the protein sequence similarities of the gyrB and rpoD genes of strain W3-3A(T) and B. denitrificans BD1(T) were 96.1 % and 91.0 %, respectively. Phylogenetic trees based on these housekeeping genes showed that strain W3-3A(T) and B. denitrificans BD1(T) formed a distinct lineage in the Gammaproteobacteria. The DNA-DNA hybridization value between strain W3-3A(T) and B. denitrificans BD1(T) was 43 %. Strain W3-3A(T) could also be differentiated from B. denitrificans BD1(T) based on the repetitive extragenic palindromic DNA-PCR fingerprint. The G+C content of the chromosomal DNA of strain W3-3A(T) was 49 mol%. The combined genotypic and phenotypic data showed that strain W3-3A(T) represents a novel species of the genus Bowmanella, for which the name Bowmanella pacifica sp. nov. is proposed, with the type strain W3-3A(T) (=CGMCC 1.7086(T)=LMG 24568(T)=MCCC 1A01018(T)).
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Gyrase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed RNA Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrenes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/RNA polymerase sigma 70,
http://linkedlifedata.com/resource/pubmed/chemical/Sigma Factor,
http://linkedlifedata.com/resource/pubmed/chemical/pyrene
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
1466-5026
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
59
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1579-82
|
|
pubmed:meshHeading |
pubmed-meshheading:19528202-Alteromonadaceae,
pubmed-meshheading:19528202-Bacterial Typing Techniques,
pubmed-meshheading:19528202-Base Composition,
pubmed-meshheading:19528202-Biodegradation, Environmental,
pubmed-meshheading:19528202-DNA, Bacterial,
pubmed-meshheading:19528202-DNA Gyrase,
pubmed-meshheading:19528202-DNA-Directed RNA Polymerases,
pubmed-meshheading:19528202-Genes, rRNA,
pubmed-meshheading:19528202-Genotype,
pubmed-meshheading:19528202-Geologic Sediments,
pubmed-meshheading:19528202-Molecular Sequence Data,
pubmed-meshheading:19528202-Nucleic Acid Hybridization,
pubmed-meshheading:19528202-Pacific Ocean,
pubmed-meshheading:19528202-Phenotype,
pubmed-meshheading:19528202-Phylogeny,
pubmed-meshheading:19528202-Pyrenes,
pubmed-meshheading:19528202-RNA, Ribosomal, 16S,
pubmed-meshheading:19528202-Sequence Analysis, DNA,
pubmed-meshheading:19528202-Sigma Factor,
pubmed-meshheading:19528202-Species Specificity
|
|
pubmed:year |
2009
|
|
pubmed:articleTitle |
Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium.
|
|
pubmed:affiliation |
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
