Linked Life Data

19514841

Source:http://linkedlifedata.com/resource/pubmed/id/19514841

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-7-31
pubmed:abstractText
Most of our knowledge of helical cytokine-like molecules in invertebrates relies on functional assays and similarities at the physicochemical level. It is hard to predict helical cytokines in invertebrates based on sequences from mammals and vertebrates, because of their long evolutionary divergence. In this article, we collected 12 kinds of fish cytokines and constructed their respective consensus sequences using hidden Markov models; then, the conserved domains region of each consensus sequence were further extracted by the SMART tool, and used as the query sequence for PSI-BLAST analysis in Drosophila melanogaster. After two filtering processes based on the properties of helical cytokines, we obtained one protein named CG14629, which shares 25% identities/46% positives to fish M17 cytokine in the half length of the N-terminus. Considering the homology between M17 and LIF/CNTF (leukemia inhibitory factor/ciliary neurotrophic factor), and the close relationship between Dome, the putative cytokine receptor in Drosophila cells, and LIFR/CNTFR (LIF receptor/CNTF receptor), the results suggest that CG14629 is a good candidate for the helical cytokine ortholog in D. melanogaster.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1557-7465
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
461-8
pubmed:meshHeading
pubmed-meshheading:19514841-Animals, pubmed-meshheading:19514841-Ciliary Neurotrophic Factor, pubmed-meshheading:19514841-Computational Biology, pubmed-meshheading:19514841-Computer Simulation, pubmed-meshheading:19514841-Consensus Sequence, pubmed-meshheading:19514841-Cytokines, pubmed-meshheading:19514841-Drosophila Proteins, pubmed-meshheading:19514841-Drosophila melanogaster, pubmed-meshheading:19514841-Evolution, Molecular, pubmed-meshheading:19514841-Fish Proteins, pubmed-meshheading:19514841-Fishes, pubmed-meshheading:19514841-Protein Structure, Secondary, pubmed-meshheading:19514841-Receptor, Ciliary Neurotrophic Factor, pubmed-meshheading:19514841-Receptors, Interleukin, pubmed-meshheading:19514841-Sequence Alignment, pubmed-meshheading:19514841-Structural Homology, Protein, pubmed-meshheading:19514841-Structure-Activity Relationship
pubmed:year
2009
pubmed:articleTitle
Identification of a putative invertebrate helical cytokine similar to the ciliary neurotrophic factor/leukemia inhibitory factor family by PSI-BLAST-based approach.
pubmed:affiliation
School of Life Science, Fudan University, Shanghai, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't