Linked Life Data

19508199

Source:http://linkedlifedata.com/resource/pubmed/id/19508199

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-3-23
pubmed:abstractText
he photo-switchable insect kinin thioxo-analog Phe(1)-Tyr(2)-Psi[CS-N]-Pro(3)-Trp(4)-Gly(5)-NH(2) (Psi[CS-N](2)-Kinin) can change from ground state to photo-stationary state by following a pulse of UV Radiation and its bioactivity increases simultaneously. To investigate the conformation-activity relationship, the solution structure of its ground state was determined by two-dimensional NMR spectroscopy. In contrast, the photo-stationary state could not be determined because of its relatively fast thermal reisomerization. The molecular dynamics-calculated structures based on NMR constraints demonstrate that the trans Pro conformer is the predominant conformation for the ground state in aqueous solution, which was also confirmed by the very weak signal of the cis Pro conformer in the spectroscopy. The aromatic side chains of residues 2 and 4 form an electrostatic interaction rather than 1 and 4. The results explain the low bioactivity before UV Radiation, and indicate the importance of the 1-4 electrostatic interaction for the activity of insect kinins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1875-5305
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Solution structure of a photo-switchable insect kinin thioxo-analog.
pubmed:affiliation
Institute of Biochemistry and Molecular Biology, School of Life Sciences, Lanzhou University, 222 Tianshui South Road, Lanzhou 730000, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't