Source:http://linkedlifedata.com/resource/pubmed/id/19502320
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 6
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pubmed:dateCreated |
2009-6-8
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pubmed:databankReference | |
pubmed:abstractText |
A novel Gram-negative, non-sporulating, moderately halophilic, facultatively alkaliphilic, catalase- and oxidase-positive, obligately aerobic bacterium, strain YIM-Y25(T), was isolated from a subterranean brine sample collected from a salt mine in Yunnan, south-west China. Cells were spirilla, motile by monopolar flagella, with meso-diaminopimelic acid in the cell-wall peptidoglycan. Growth occurred with 1-15 % (w/v) NaCl (optimum 5 %), at pH 6.0-10.0 (optimum pH 8.0) and at 15-50 degrees C (optimum 35-40 degrees C). Ubiquinone Q-8 was the predominant respiratory quinone. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylamine and an unidentified phospholipid. The major cellular fatty acids were C(18 : 1)omega7c, iso-C(16 : 0), C(16 : 0) and C(16 : 1). The genomic DNA G+C content was 58.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM-Y25(T) was most closely related to the type strain of the sole recognized species of the genus Saccharospirillum, Saccharospirillum impatiens EL-105(T) (sequence similarity 97.0 %), and these two strains formed a robust lineage in the phylogenetic tree. The level of DNA-DNA relatedness between them was 12.6 %. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic properties and DNA-DNA hybridization data supported the view that this strain represents a novel species of the genus Saccharospirillum, for which the name Saccharospirillum salsuginis sp. nov. is proposed, with YIM-Y25(T) (=CCTCC AA 207033(T) =KCTC 22169(T)) as the type strain.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1466-5026
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1382-6
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pubmed:meshHeading |
pubmed-meshheading:19502320-Bacterial Typing Techniques,
pubmed-meshheading:19502320-Base Composition,
pubmed-meshheading:19502320-China,
pubmed-meshheading:19502320-DNA, Bacterial,
pubmed-meshheading:19502320-DNA, Ribosomal,
pubmed-meshheading:19502320-Fatty Acids,
pubmed-meshheading:19502320-Gammaproteobacteria,
pubmed-meshheading:19502320-Molecular Sequence Data,
pubmed-meshheading:19502320-Nucleic Acid Hybridization,
pubmed-meshheading:19502320-Phenotype,
pubmed-meshheading:19502320-Phylogeny,
pubmed-meshheading:19502320-RNA, Ribosomal, 16S,
pubmed-meshheading:19502320-Sequence Analysis, DNA,
pubmed-meshheading:19502320-Sodium Chloride,
pubmed-meshheading:19502320-Soil Microbiology,
pubmed-meshheading:19502320-Species Specificity
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pubmed:year |
2009
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pubmed:articleTitle |
Saccharospirillum salsuginis sp. nov., a gammaproteobacterium from a subterranean brine.
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pubmed:affiliation |
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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