Source:http://linkedlifedata.com/resource/pubmed/id/19450710
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2009-9-7
|
| pubmed:abstractText |
Transforming growth factor-beta (TGF-beta) can induce G2/M phase-dependent apoptosis and G1/S phase-dependent epithelial-mesenchymal transition (EMT) in hepatocytes, but the underlying mechanism remains poorly understood. In this study, we investigated alterations in the global proteome using two dimensional gel electrophoresis of AML-12 murine hepatocyte cells after treatment with TGF-beta at several time points after synchronization in the G2/M or G1/S phase. Upon TGF-beta treatment, the expression levels of 44 proteins were found to be significantly changed in cells synchronized in the G2/M phase. These proteins were identified by MALDI-TOF/TOF and classified into seven categories according to function. In addition, TGF-beta induced downregulation of glutamine synthetase in cells in G2/M but not G1/S phase, and this was further confirmed by immunoblotting. Moreover, exogenous glutamine completely blocked TGF-beta-induced apoptosis in G2/M and non-synchronized cells, whereas it had no effect on EMT, suggesting that the downregulation of glutamine synthetase is involved in G2/M phase-dependent apoptosis. These results provide new insight into the mechanism of the multifunctional effects of TGF-beta and how apoptosis and EMT are regulated in the same type of cells.
|
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0006-3002
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
1794
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1387-97
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| pubmed:meshHeading |
pubmed-meshheading:19450710-Animals,
pubmed-meshheading:19450710-Apoptosis,
pubmed-meshheading:19450710-Cell Cycle,
pubmed-meshheading:19450710-Cell Line,
pubmed-meshheading:19450710-DNA,
pubmed-meshheading:19450710-Down-Regulation,
pubmed-meshheading:19450710-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:19450710-Epithelial Cells,
pubmed-meshheading:19450710-Glutamate-Ammonia Ligase,
pubmed-meshheading:19450710-Hepatocytes,
pubmed-meshheading:19450710-Membrane Potential, Mitochondrial,
pubmed-meshheading:19450710-Mesoderm,
pubmed-meshheading:19450710-Mice,
pubmed-meshheading:19450710-Proteome,
pubmed-meshheading:19450710-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:19450710-Tandem Mass Spectrometry,
pubmed-meshheading:19450710-Transforming Growth Factor beta
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
Comparative proteomic analysis of cell cycle-dependent apoptosis induced by transforming growth factor-beta.
|
| pubmed:affiliation |
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|