Linked Life Data

19448036

Source:http://linkedlifedata.com/resource/pubmed/id/19448036

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2009-8-6
pubmed:abstractText
The pepper (Capsicum annuum) bacterial spot (Bs) resistance gene Bs3 and its allelic variant Bs3-E mediate recognition of the Xanthomonas campestris pv vesicatoria type III effector protein AvrBs3 and its deletion derivative AvrBs3Deltarep16. Recognition specificity resides in the Bs3 and Bs3-E promoters and is determined by a defined promoter region, the UPA (for up-regulated by AvrBs3) box. Using site-directed mutagenesis, we defined the exact boundaries of the UPA(AvrBs3) box of the Bs3 promoter and the UPA(AvrBs3Deltarep16) box of the Bs3-E promoter and show that both boxes overlap by at least 11 nucleotides. Despite partial sequence identity, the UPA(AvrBs3) box and the UPA(AvrBs3Deltarep16) box were bound specifically by the corresponding AvrBs3 and AvrBs3Deltarep16 proteins, respectively, suggesting that selective promoter binding of AvrBs3-like proteins is the basis for promoter activation specificity. We also demonstrate that the UPA(AvrBs3) box retains its functionality at different positions within the pepper Bs3 promoter and confers AvrBs3 inducibility in a novel promoter context. Notably, the transfer of the UPA(AvrBs3) box to different promoter locations is always correlated with a new transcriptional start site. The analysis of naturally occurring Bs3 alleles revealed many pepper accessions that encode a nonfunctional Bs3 variant. These accessions showed no apparent abnormalities, supporting the supposition that Bs3 functions only in disease resistance and not in other developmental or physiological processes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-10931960, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-11209081, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-11332727, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-11439138, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-11992820, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-14675431, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-14764104, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-15020460, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-15071555, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-15862106, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-15973413, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-16353556, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-16403589, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-16448329, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-16497589, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-16648463, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-16753033, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-16798873, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-16936700, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-17108957, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-17506648, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-17563377, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-17697981, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-17765596, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-17885085, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-17962564, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-17962565, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-18422429, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-18657470, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-19086184, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-19132864, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-19154004, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-19168384, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-19168386, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-2550761, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-6100894, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-6283634, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-8980236, http://linkedlifedata.com/resource/pubmed/commentcorrection/19448036-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
150
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1697-712
pubmed:dateRevised
2010-9-27
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Recognition of AvrBs3-like proteins is mediated by specific binding to promoters of matching pepper Bs3 alleles.
pubmed:affiliation
Institute of Biology, Department of Genetics , Martin Luther University Halle-Wittenberg, 06120 Halle , Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't